FcZero-rAb® PerCP-Cyanine5.5 Anti-6*His, His-Tag Rabbit Recombinant Antibody

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• FcZero-rAb® PerCP-Cyanine5.5 Anti-6*His, His-Tag Rabbit Recombinant Antibody (CPY5-FcA98450)

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  FC experiment of Transfected HEK-293T using CPY5-FcA98450

  1x10^6 transfected HEK-293T cells or unransfected HEK-293T cells were intracellularly stained with 0.25 ug PerCP-Cyanine5.5 Anti-6*His, His-Tag Rabbit RecAb (CPY5-FcA98450, Clone: 251107A1). Cells were incubated with MonoZero™ Monocytes blocking Reagent (PF00020) prior to staining. Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

• 

  FC experiment of Transfected HEK-293T using CPY5-FcA98450

  1x10^6 transfected HEK-293T cells were intracellularly stained with 0.25 ug PerCP-Cyanine5.5 Anti-6*His, His-Tag Rabbit RecAb (CPY5-FcA98450, Clone: 251107A1) (red) or FcZero-rAb™ PerCP-Cyanine5.5 Rabbit IgG Isotype Control Recombinant Antibody (CPY5-FcA98136, Clone: 240953C9) (blue). 1x10^6 untransfected HEK-293T cells were intracellularly stained with 0.25 ug PerCP-Cyanine5.5 Anti-6*His, His-Tag Rabbit RecAb (CPY5-FcA98450, Clone: 251107A1) (black,dashed). Cells were incubated with MonoZero™ Monocytes blocking Reagent (PF00020) prior to staining. Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

6*His, His-Tag FcZero-rAb^® Recombinant Antibody for FC (Intra)

Cat No. CPY5-FcA98450

Clone No.251107A1

Host / Isotype

Rabbit / IgG

Reactivity

n/a

Applications

FC (Intra)

Synonyms

6*His, His Tag, His Tag, 6*His, 6*his tag, 6*his,His tag

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Tested Applications

• Positive FC (Intra) detected in: Transfected HEK-293T cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.1 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CPY5-FcA98450 targets 6*His, His-Tag in FC (Intra) applications and shows reactivity with n/a samples.

• Tested Reactivity: n/a
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  unknow

• Full Name: 6*His, His-Tag
• Calculated Molecular Weight: 1 kDa
• RRID: AB_3749248
• Conjugate: PerCP-Cyanine5.5 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 482 nm / 690nm
• Excitation Laser: Blue laser (488 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: HISTAG
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His-tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His-tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.