"MBP tag Antibodies" Comparison
View side-by-side comparison of MBP tag antibodies from other vendors to find the one that best suits your research needs.
Tested Applications
| Positive WB detected in | Recombinant protein, transfected HEK-293T cells |
| Positive IP detected in | Recombinant protein protein |
| Positive IF/ICC detected in | Transfected cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66003-1-Ig targets MBP tag in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with recombinant protein samples.
| Tested Reactivity | recombinant protein |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0942 Product name: Recombinant MBP-Tag protein Source: e coli.-derived, Unknow Tag: N/A Sequence: Predict reactive species |
| Full Name | MBP tag |
| Calculated Molecular Weight | 40 kDa |
| Observed Molecular Weight | 40 kDa |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_11183040 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein(MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by Western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. An antibody to MBP can also be used to isolate or detect expression of the protein.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for MBP tag antibody 66003-1-Ig | Download protocol |
| WB protocol for MBP tag antibody 66003-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MBP tag Monoclonal antibody (4C6H4), catalog number 66003-1-Ig, has accumulated 105 citations. Publications appear in high-impact journals including Nature, Science, Cell, Nature Communications, Molecular Cell, Science Advances, Plant Cell, Current Biology, and EMBO Journal, among others. Research fields span plant biology and agriculture (stress tolerance, immunity, development), neuroscience (Alzheimer's disease, neurodegeneration), virology (Zika, influenza, SARS-CoV-2), autophagy, cancer biology, and general cell biology.
| Species | Application | Title |
|---|---|---|
Autophagy The Valsa mali effector Vm1G-1794 protects the aggregated MdEF-Tu from autophagic degradation to promote infection in apple | ||
Autophagy GORASP2 promotes phagophore closure and autophagosome maturation into autolysosomes |
Reviews
What customers say
One reviewer (Daniel U., August 2025) gave this antibody a perfect 5-star rating after using it at a 1:10,000 dilution in Western Blot to detect recombinant MBP protein. He reported that the antibody successfully detected as little as 50 ng of recombinant MBP and described the signal as "very strong." Overall, the single review reflects a highly positive experience with strong, reliable detection performance.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Daniel (Verified Customer) (08-07-2025) | Antibody successfully detected 50 ng recombinant MBP protein. The signal was very strong!
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