MAVS; VISA Monoclonal antibody

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• MAVS; VISA Monoclonal antibody (66911-1-Ig)

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• KD/KO Validated

MAVS; VISA Monoclonal Antibody for WB, IHC, ELISA

Cat No. 66911-1-Ig Copied!

Clone No. 1A8E9

Publications (22)

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Host / Isotype

Mouse / IgG1

Reactivity

human and More (3)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

MAVS, 1A8E9, Cardif, IPS 1, IPS1

• 

  WB analysis of A431 using 66911-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 66911-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 66911-1-Ig

  WB result of MAVS; VISA antibody (66911-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MAVS; VISA transfected HeLa cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of L02 using 66911-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Jurkat using 66911-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 66911-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 66911-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 66911-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:1100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 66911-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:1100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, HepG2 cells, Jurkat cells, L02 cells, HEK-293 cells, THP-1cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:550-1:2200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66911-1-Ig targets MAVS; VISA in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, pig, monkey
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5949

  Product name: Recombinant human MAVS; VISA protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-347 aa of BC044952

  Sequence: MPFAEDKTYKYICRNFSNFCNVDVVEILPYLPCLTARDQDRLRATCTLSGNRDTLWHLFNTLQRRPGWVEYFIAALRGCELVDLADEVASVYESYQPRTSDRPPDPLEPPSLPAERPGPPTPAAAHSIPYNSCREKEPSYPMPVQETQAPESPGENSEQALQTLSPRAIPRNPDGGPLESSSDLAALSPLTSSGHQEKDTELGSTHTAGATSSLTPSRGPVSPSVSFQPLARSTPRASRLPGPTGSVVSTGTSFSSSSPGLASAGAAEGKQGAESDQAEPIICSSGAEAPANSLPSKVPTTLMPVNTVALKVPANPASVSTVPSKLPTSSKPPGAVPSNALTNPAPS

• Full Name: mitochondrial antiviral signaling protein
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 50-55 kDa, 70-75 kDa
• GenBank Accession Number: BC044952
• Gene Symbol: MAVS
• Gene ID (NCBI): 57506
• RRID: AB_2882238
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q7Z434
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mitochondrial antiviral-signaling protein (MAVS) is also known as virus-induced-signaling adapter (VISA) or IFN beta promoter stimulator protein 1 (IPS-1), it is widely involved and required for innate immune defense against viruses. MAVS, present in T cells, monocytes, epithelial cells and hepatocytes, contains CARD and transmembrane domains which are essential for antiviral functions. MAVS is able to interact with various cellular proteins including DDX58/RIG-I, IFIH1/MDA5, TRAF2, TRAF6, TMEM173/MITA, IFIT3 and etc. It can undergoe phosphorylation on multiple sites and ubiquitination, which may together cause the molecular weight migrate to about 70 kDa despite the predicated 57 kDa.

Publications

What published studies show

MAVS antibody (Cat# 66911-1-Ig) has accumulated 22 citations across 19 journals including Autophagy, Molecular Cell, Advanced Science, Cell Death & Differentiation, Nature Communications, Journal of Virology, and PLoS Pathogens. Research fields span viral innate immunity (bunyavirus, influenza, enterovirus, coronavirus), autophagy-mediated immune evasion, cancer immunotherapy (ovarian, lung, glioma), neuroinflammation, and STING/RIG-I signaling pathways.

Automatically generated from the 22 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human		Autophagy; Bunyavirus SFTSV NSs utilizes autophagy to escape the antiviral innate immune response; Authors - Ze-Min Li
monkey,human	WB,IF,CoIP	Autophagy; Bunyavirus SFTSV nucleoprotein exploits tufm-mediated mitophagy to impair antiviral innate immunity; Authors - Wen-Kang Zhang
	IF	Mol Cell; An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation; Authors - Stephanie Pei Tung Yiu
mouse	WB	Adv Sci (Weinh); Carnosic Acid Directly Targets STING C-Terminal Tail to Improve STING-Mediated Inflammatory Diseases; Authors - Wenqing Mu
human	WB	Cell Death Differ; Targeting ADAR1 lactylation activates innate immune and overcomes chemoresistance in ovarian cancer.; Authors - Linlu Cui
mouse,human	WB	J Immunother Cancer; Targeting KDM4C enhances CD8+ T cell mediated antitumor immunity by activating chemokine CXCL10 transcription in lung cancer.; Authors - Xiaohua Jie