Alpha-2-Macroglobulin Monoclonal antibody

Alpha-2-Macroglobulin Monoclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

4B11F7

Cat no : 66126-1-Ig

Synonyms

A2M, Alpha 2 M, alpha 2 macroglobulin, Alpha-2-Macroglobulin, CPAMD5, FWP007, S863 7, α-2M



Tested Applications

Positive WB detected inHepG2 cells, human brain tissue
Positive IP detected inhuman plasma tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 66126-1-Ig is Alpha-2-Macroglobulin Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Alpha-2-Macroglobulin fusion protein Ag19245
Full Name alpha-2-macroglobulin
Calculated Molecular Weight 1474 aa, 163 kDa
Observed Molecular Weight 185 kDa
GenBank Accession NumberBC040071
Gene Symbol A2M
Gene ID (NCBI) 2
RRIDAB_2881525
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

alpha-2-macroglobulin, also known as α2-macroglobulin (α2M and A2M), is a protein abundant in the plasma of vertebrates and several invertebrates. A2M is an evolutionarily conserved arm of the innate immune system. It also mediates the proliferation of T cells and macrophages. A2M acts as a nonspecific protease inhibitor involved in the host defense mechanism that inactivates both endogenous and exogenous proteases, including trypsin, thrombin and collagenase. Even though A2M is produced predominantly by the liver, it may also be expressed in the reproductive tract, heart, and brain, and may have important roles in many physiological processes and medical illnesses including Alzheimer's disease.

Protocols

Product Specific Protocols
WB protocol for Alpha-2-Macroglobulin antibody 66126-1-IgDownload protocol
IHC protocol for Alpha-2-Macroglobulin antibody 66126-1-IgDownload protocol
IF protocol for Alpha-2-Macroglobulin antibody 66126-1-IgDownload protocol
IP protocol for Alpha-2-Macroglobulin antibody 66126-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols