CoraLite® Plus 488-conjugated Alpha-2-Macroglobulin Monoclonal antibody

Alpha-2-Macroglobulin Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4B11F7

Cat no : CL488-66126

Synonyms

A2M, Alpha 2 M, alpha 2 macroglobulin, Alpha-2-Macroglobulin, CPAMD5, DKFZp779B086, FWP007, S863 7

---

Validation Data Gallery

• 

  IF Staining of HepG2 using CL488-66126

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL488-66126 (Alpha-2-macroglobulin antibody) at dilution of 1:100.

• 

  IF Staining of HepG2 using CL488-66126

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 Alpha-2-Macroglobulin antibody (CL488-66126, Clone: 4B11F7 ) at dilution of 1:200.

---

Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-66126 is Alpha-2-Macroglobulin Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Alpha-2-Macroglobulin fusion protein Ag19245
• Full Name: alpha-2-macroglobulin
• Calculated Molecular Weight: 1474 aa, 163 kDa
• GenBank Accession Number: BC040071
• Gene Symbol: Alpha 2 Macroglobulin
• Gene ID (NCBI): 2
• RRID: AB_2883267
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

alpha-2-macroglobulin, also known as α2-macroglobulin (α2M and A2M), is a protein abundant in the plasma of vertebrates and several invertebrates. A2M is an evolutionarily conserved arm of the innate immune system. It also mediates the proliferation of T cells and macrophages. A2M acts as a nonspecific protease inhibitor involved in the host defense mechanism that inactivates both endogenous and exogenous proteases, including trypsin, thrombin and collagenase. Even though A2M is produced predominantly by the liver, it may also be expressed in the reproductive tract, heart, and brain, and may have important roles in many physiological processes and medical illnesses including Alzheimer's disease.