CoraLite® Plus 488-conjugated AATF Monoclonal antibody

AATF Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2A6G6

Cat no : CL488-67868

Synonyms

AATF, CHE 1, CHE1, DED, Protein AATF, Rb binding protein Che 1, Traube

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Validation Data Gallery

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  FC experiment of HeLa using CL488-67868

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human AATF (CL488-67868, Clone:2A6G6) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive FC detected in: HeLa cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67868 targets AATF in FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: AATF fusion protein Ag0195
• Full Name: apoptosis antagonizing transcription factor
• Calculated Molecular Weight: 63 kDa
• Observed Molecular Weight: 80-90 kDa
• GenBank Accession Number: BC000591
• Gene Symbol: AATF
• Gene ID (NCBI): 26574
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Apoptosis antagonizing transcription factor (AATF), an interacting partner of RNA polymerase II is a multifunctional protein that is highly conserved in eukaryotes. In addition to nuclear hormone receptors, AATF has been shown to interact with several transcription factors, including the retinoblastoma protein (pRb), p65, and STAT3(PMID: 34785906). Furthermore AATF serves as a critical regulator in various cancers and promotes tumorigenesis by protecting cancer cells from apoptosis induction, favoring cell proliferation, or promoting cell survival by autophagy(PMID: 33145763).

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Foxp3 / Transcription Factor Staining Buffer Kit

PF00011