ABCA1 Polyclonal antibody

ABCA1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26564-1-AP

Synonyms

ABC 1, ABC1, ABCA1, ATP binding cassette 1, CERP, HDLDT1, TGD



Tested Applications

Positive WB detected inL02 cells
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26564-1-AP targets ABCA1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ABCA1 fusion protein Ag24118
Full Name ATP-binding cassette, sub-family A (ABC1), member 1
Calculated Molecular Weight 2261 aa, 254 kDa
Observed Molecular Weight250 kDa
GenBank Accession NumberBC141816
Gene Symbol ABCA1
Gene ID (NCBI) 19
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABCA, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABCA subfamily. Members of the ABCA subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. With cholesterol as its substrate, this protein functions as a cholesterol efflux pump in the cellular lipid removal pathway.

Protocols

Product Specific Protocols
WB protocol for ABCA1 antibody 26564-1-APDownload protocol
IHC protocol for ABCA1 antibody 26564-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols