ABCB8 Polyclonal antibody, PBS Only

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• ABCB8 Polyclonal antibody, PBS Only (27158-1-PBS)

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  WB analysis of MCF-7 using 27158-1-AP (same clone as 27158-1-PBS)

  WB result of ABCB8 antibody (27158-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ABCB8 transfected MCF-7 cells. This data was developed using the same antibody clone with 27158-1-PBS in a different storage buffer formulation.

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  WB analysis using 27158-1-AP (same clone as 27158-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27158-1-AP (ABCB8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27158-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 27158-1-AP (same clone as 27158-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27158-1-AP (ABCB8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27158-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 27158-1-AP (same clone as 27158-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27158-1-AP (ABCB8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27158-1-PBS in a different storage buffer formulation.

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  IF Staining of H9C2 using 27158-1-AP (same clone as 27158-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using ABCB8 antibody (27158-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27158-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

ABCB8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 27158-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ABCB 8, ATP Binding Cassette Subfamily B Member 8, ATP-binding cassette sub-family B member 8, mitochondrial, M ABC1, MABC1

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Product Information

27158-1-PBS targets ABCB8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag25935

  Product name: Recombinant human ABCB8 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 403-596 aa of BC141814

  Sequence: TVQRSMANLSVLFGQVVRGLSAGARVFEYMALNPCIPLSGGCCVPKEQLRGSVTFQNVCFSYPCRPGFEVLKDFTLTLPPGKIVALVGQSGGGKTTVASLLERFYDPTAGVVMLDGRDLRTLDPSWLRGQVVGFISQEPVLFGTTIMENIRFGKLEASDEEVYTAAREANAHEFITSFPEGYNTVVGERGTTLS

• Full Name: ATP-binding cassette, sub-family B (MDR/TAP), member 8
• Calculated Molecular Weight: 735 aa, 80 kDa
• Observed Molecular Weight: 60-65 kDa
• GenBank Accession Number: BC141814
• Gene Symbol: ABCB8
• Gene ID (NCBI): 11194
• RRID: AB_3742217
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NUT2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ABCB8 (ATP-binding cassette sub-family B member 8) is a protein located in the mitochondrial inner membrane and belongs to the ATP-binding cassette (ABC) transporter superfamily. It plays a crucial role in maintaining mitochondrial iron homeostasis and the maturation of cellular iron-sulfur cluster proteins. ABCB8 is essential for normal cardiac function by promoting the export of iron from mitochondria. Additionally, ABCB8 forms a complex with the mitochondrial KATP channel, protecting cells from oxidative stress damage. The loss of function or abnormal expression of ABCB8 is associated with various diseases, including cardiomyopathy and iron metabolism disorders.