MRP1 Monoclonal antibody
MRP1 Monoclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human and More (1)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1G4A2
Cat no : 67228-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | unboiled HepG2 cells, unboiled A549 cells |
Positive IF/ICC detected in | A549 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 13 publications below |
IHC | See 2 publications below |
Product Information
67228-1-Ig targets MRP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human, mouse |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | MRP1 fusion protein Ag27048 |
Full Name | ATP-binding cassette, sub-family C (CFTR/MRP), member 1 |
Calculated Molecular Weight | 172 kDa |
Observed Molecular Weight | 180-190 kDa |
GenBank Accession Number | NM_004996 |
Gene Symbol | MRP1 |
Gene ID (NCBI) | 4363 |
RRID | AB_2882516 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Multidrug resistant-associated protein 1 (MRP1; ABCC1) is a member of the ATP-binding cassette (ABC) transporter protein superfamily, subfamily C. MRP1 is overexpressed in cancers and contributes to the occurrence of multidrug resistance (MDR). Expression of MRP1 has been considered as a negative marker for the outcome of chemotherapy. Recently MRP1 has been reported to play a crucial role in Aβ clearance at the blood-brain barrier.
Protocols
Product Specific Protocols | |
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WB protocol for MRP1 antibody 67228-1-Ig | Download protocol |
IF protocol for MRP1 antibody 67228-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Environ Pollut Biotransformation of graphene oxide within lung fluids could intensify its synergistic biotoxicity effect with cadmium by inhibiting cellular efflux of cadmium. | ||
Cancers (Basel) NCAPG2 Maintains Cancer Stemness and Promotes Erlotinib Resistance in Lung Adenocarcinoma | ||
Front Cell Dev Biol Apatinib Suppresses Gastric Cancer Stem Cells Properties by Inhibiting the Sonic Hedgehog Pathway. | ||
Transl Oncol Pan-cancer analysis of ABCC1 as a potential prognostic and immunological biomarker | ||