Tested Applications
| Positive WB detected in | unboiled HepG2 cells, unboiled A549 cells |
| Positive IHC detected in | human appendicitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67228-1-Ig targets MRP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag27048 Product name: Recombinant human ABCC1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 918-1025 aa of NM_004996 Sequence: SSYSGDISRHHNSTAELQKAEAKKEETWKLMEADKAQTGQVKLSVYWDYMKAIGLFISFLSIFLFMCNHVSALASNYWLSLWTDDPIVNGTQEHTKVRLSVYGALGIS Predict reactive species |
| Full Name | ATP-binding cassette, sub-family C (CFTR/MRP), member 1 |
| Calculated Molecular Weight | 172 kDa |
| Observed Molecular Weight | 180-190 kDa |
| GenBank Accession Number | NM_004996 |
| Gene Symbol | MRP1 |
| Gene ID (NCBI) | 4363 |
| RRID | AB_2882516 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P33527 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Multidrug resistant-associated protein 1 (MRP1; ABCC1) is a member of the ATP-binding cassette (ABC) transporter protein superfamily, subfamily C. MRP1 is overexpressed in cancers and contributes to the occurrence of multidrug resistance (MDR). Expression of MRP1 has been considered as a negative marker for the outcome of chemotherapy. Recently MRP1 has been reported to play a crucial role in Aβ clearance at the blood-brain barrier.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MRP1 antibody 67228-1-Ig | Download protocol |
| IHC protocol for MRP1 antibody 67228-1-Ig | Download protocol |
| WB protocol for MRP1 antibody 67228-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
MRP1/ABCC1 Monoclonal Antibody (1G4A2), Cat# 67228-1-Ig, has 26 citations across journals including Cell Death & Disease, Pharmacological Research, International Journal of Nanomedicine, NPJ Breast Cancer, Environmental Pollution, Communications Chemistry, Biochemical Pharmacology, Food & Function, Pharmaceuticals, Cancers, Journal of Pathology, Frontiers in Pharmacology, Translational Oncology, and American Journal of Cancer Research. Research fields encompass cancer biology, multidrug resistance, chemoresistance, ferroptosis, drug development, and environmental toxicology.
| Species | Application | Title |
|---|---|---|
Cell Death Dis PLSCR1 drives chemoresistance in TNBC via METTL3/IGF2BP3-mediated mRNA stabilization and EGFR-MAPK pathway activation. | ||
Cell Death Dis Cancer-associated fibroblasts-mediated ATF4 expression promotes malignancy and gemcitabine resistance in pancreatic cancer via the TGF-β1/SMAD2/3 pathway and ABCC1 transactivation. | ||
Pharmacol Res Identification of Fasudil as a collaborator to promote the anti-tumor effect of lenvatinib in hepatocellular carcinoma by inhibiting GLI2-mediated hedgehog signaling pathway | ||
Int J Nanomedicine SiRNF8 Delivered by DNA Framework Nucleic Acid Effectively Sensitizes Chemotherapy in Colon Cancer | ||
NPJ Breast Cancer Mechanisms of resistance to trastuzumab deruxtecan in breast cancer elucidated by multi-omic molecular profiling. | ||
Environ Pollut Biotransformation of graphene oxide within lung fluids could intensify its synergistic biotoxicity effect with cadmium by inhibiting cellular efflux of cadmium. |
