Tested Applications
| Positive WB detected in | HepG2 cells, A431 cells, U2OS cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67960-1-Ig targets ABCE1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28493 Product name: Recombinant human ABCE1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 76-256 aa of BC016283 Sequence: PSNLEKETTHRYCANAFKLHRLPIPRPGEVLGLVGTNGIGKSTALKILAGKQKPNLGKYDDPPDWQEILTYFRGSELQNYFTKILEDDLKAIIKPQYVDQIPKAAKGTVGSILDRKDETKTQAIVCQQLDLTHLKERNVEDLSGGELQRFACAVVCIQKADIFMFDEPSSYLDVKQRLKAA Predict reactive species |
| Full Name | ATP-binding cassette, sub-family E (OABP), member 1 |
| Calculated Molecular Weight | 599 aa, 67 kDa |
| Observed Molecular Weight | 68 kDa |
| GenBank Accession Number | BC016283 |
| Gene Symbol | ABCE1 |
| Gene ID (NCBI) | 6059 |
| RRID | AB_2918711 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P61221 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ABCE1 is an ATPase that is a member of the ATP-binding cassette (ABC) transporters superfamily and OABP subfamily, it is an essential and highly conserved protein that is required for both eukaryotic translation initiation as well as ribosome biogenesis.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ABCE1 antibody 67960-1-Ig | Download protocol |
| IHC protocol for ABCE1 antibody 67960-1-Ig | Download protocol |
| WB protocol for ABCE1 antibody 67960-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Daniel (Verified Customer) (10-26-2022) | This antibody does not work in any cell extract tested. It doesn't work in PVDF or nitrocellulose, multiple dilution and different blocking buffers.
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