ABHD14B Polyclonal antibody

ABHD14B Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 20952-1-AP

Synonyms

ABHD14B, CCG1 interacting factor B, CIB

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Validation Data Gallery

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  WB analysis of L02 using 20952-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20952-1-AP (ABHD14B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 20952-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 20952-1-AP (ABHD14B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate hyperplasia using 20952-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 20952-1-AP (ABHD14B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate hyperplasia using 20952-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 20952-1-AP (ABHD14B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 20952-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 20952-1-AP (ABHD14B antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 20952-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 20952-1-AP (ABHD14B antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 20952-1-AP

  Immunofluorescent analysis of HepG2 cells, using ABHD14B antibody 20952-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: L02 cells, mouse small intestine tissue
• Positive IHC detected in: human prostate hyperplasia tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20952-1-AP is ABHD14B Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ABHD14B fusion protein Ag15097
• Full Name: abhydrolase domain containing 14B
• Calculated molecular weight: 210 aa, 22 kDa
• Observed molecular weight: 22-25 kDa
• GenBank accession number: BC007234
• Gene symbol: ABHD14B
• Gene ID (NCBI): 84836
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.