ABHD14B Polyclonal antibody
ABHD14B Polyclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF,ELISA
Conjugate
Unconjugated
Cat no : 20952-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | L02 cells, mouse small intestine tissue |
Positive IHC detected in | human prostate hyperplasia tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2400 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Product Information
The immunogen of 20952-1-AP is ABHD14B Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ABHD14B fusion protein Ag15097 |
Full Name | abhydrolase domain containing 14B |
Calculated molecular weight | 210 aa, 22 kDa |
Observed molecular weight | 22-25 kDa |
GenBank accession number | BC007234 |
Gene symbol | ABHD14B |
Gene ID (NCBI) | 84836 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for ABHD14B antibody 20952-1-AP | Download protocol |
IHC protocol for ABHD14B antibody 20952-1-AP | Download protocol |
IF protocol for ABHD14B antibody 20952-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |