ABI3 Polyclonal antibody

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  WB analysis using 16227-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16227-1-AP (ABI3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 16227-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16227-1-AP (ABI3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 16227-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16227-1-AP (ABI3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 16227-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using ABI3 antibody (16227-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

ABI3 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16227-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

SSH3BP3, New molecule including SH3, Nesh, ABI gene family member 3, ABI family, member 3

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Tested Applications

• Positive WB detected in: HUVEC cells, SK-N-SH cells
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16227-1-AP targets ABI3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8164

  Product name: Recombinant human ABI3 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-366 aa of BC007780

  Sequence: MAELQQLQEFEIPTGREALRGNHSALLRVADYCEDNYVQATDKRKALEETMAFTTQALASVAYQVGNLAGHTLRMLDLQGAALRQVEARVSTLGQMVNMHMEKVARREIGTLATVQRLPPGQKVIAPENLPPLTPYCRRPLNFGCLDDIGHGIKDLSTQLSRTGTLSRKSIKAPATPASATLGRPPRIPEPVHLPVVPDGRLSAASSASSLASAGSAEGVGGAPTPKGQAAPPAPPLPSSLDPPPPPAAVEVFQRPPTLEELSPPPPDEELPLPLDLPPPPPLDGDELGLPPPPPGFGPDEPSWVPASYLEKVVTLYPYTSQKDNELSFSEGTVICVTRRYSDGWCEGVSSEGTGFFPGNYVEPSC

• Full Name: ABI family, member 3
• Calculated Molecular Weight: 366 aa, 39 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC007780
• Gene Symbol: ABI3
• Gene ID (NCBI): 51225
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9P2A4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ABI family member 3 belongs to the ABL Interactors (ABI) family of proteins, which also includes ABI1 and ABI2. ABI proteins are a class of cytoplasmic molecular junction proteins originally identified as binding chaperones for Abl kinase. The ABI3 gene is thought to be an important regulator of microglia during Alzheimer's disease (AD), and its S209F variant increases the risk of developing AD. Moreover, ABI3 plays an important role in suppressing tumor growth and progression.