STARS/ABRA Polyclonal antibody

STARS/ABRA Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22673-1-AP

Synonyms

ABRA, STARS



Tested Applications

Positive WB detected inmouse heart tissue, human heart tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22673-1-AP is STARS/ABRA Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STARS/ABRA fusion protein Ag18456
Full Name actin-binding Rho activating protein
Calculated molecular weight 381 aa, 43 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC105103
Gene symbol ABRA
Gene ID (NCBI) 137735
RRIDAB_11182712
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for STARS/ABRA antibody 22673-1-APDownload protocol
IHC protocol for STARS/ABRA antibody 22673-1-APDownload protocol
IF protocol for STARS/ABRA antibody 22673-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Development

HAND2-mediated epithelial maintenance and integrity in cardiac outflow tract morphogenesis.

Authors - Meng Xia
mouseWB

Behav Brain Res

Changes in mRNA and miRNA expression in the prelimbic cortex related to depression-like syndrome induced by chronic social defeat stress in mice

Authors - Yexiang Chen
human,mouseWB,IF

Am J Pathol

Different mouse models of nemaline myopathy harboring Acta1 mutations display differing abnormalities related to mitochondrial biology

Authors - Jennifer A Tinklenberg