ACAD9 Polyclonal antibody, PBS Only

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• ACAD9 Polyclonal antibody, PBS Only (15770-1-PBS)

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  WB analysis using 15770-1-AP (same clone as 15770-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15770-1-AP (ACAD9 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15770-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 15770-1-AP (same clone as 15770-1-PBS)

  WB result of ACAD9 antibody (15770-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACAD9 transfected MCF-7 cells. This data was developed using the same antibody clone with 15770-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 15770-1-AP (same clone as 15770-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15770-1-AP (ACAD9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15770-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 15770-1-AP (same clone as 15770-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15770-1-AP (ACAD9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15770-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

ACAD9 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 15770-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

ACAD 9, ACAD-9, Acyl-CoA dehydrogenase family member 9, Complex I assembly factor ACAD9, mitochondrial, EC:1.3.8.-

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Product Information

15770-1-PBS targets ACAD9 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8414

  Product name: Recombinant human ACAD9 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 285-621 aa of BC007970

  Sequence: ILGEVGDGFKVAMNILNSGRFSMGSVVAGLLKRLIEMTAEYACTRKQFNKRLSEFGLIQEKFALMAQKAYVMESMTYLTAGMLDQPGFPDCSIEAAMVKVFSSEAAWQCVSEALQILGGLGYTRDYPYERILRDTRILLIFEGTNEILRMYIALTGLQHAGRILTTRIHELKQAKVSTVMDTVGRRLRDSLGRTVDLGLTGNHGVVHPSLADSANKFEENTYCFGRTVETLLLRFGKTIMEEQLVLKRVANILINLYGMTAVLSRASRSIRIGLRNHDHEVLLANTFCVEAYLQNLFSLSQLDKYAPENLDEQIKKVSQQILEKRAYICAHPLDRTC

• Full Name: acyl-Coenzyme A dehydrogenase family, member 9
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 60-65 kDa
• GenBank Accession Number: BC007970
• Gene Symbol: ACAD9
• Gene ID (NCBI): 28976
• RRID: AB_2241997
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H845
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Acyl-CoA dehydrogenases (ACADs) are a family of mitochondrial enzymes catalyzing the initial rate-limiting step in the β-oxidation of fatty acyl-CoA. ACAD9 belongs to the group of ACADs. The deduced 621-amino acid protein has a calculated molecular mass of 68.8 kD. It has an N-terminal leader sequence, 2 conserved motifs shared by all ACAD family members, and a potential N-glycosylation site(PMID:12359260). Defects in ACAD9 are a cause of acyl-CoA dehydrogenase family member type 9 deficiency (ACAD9 deficiency).