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ACADS Polyclonal antibody, PBS Only (16623-1-PBS)

Filter: AllWBIPIHC

WB analysis using 16623-1-AP (same clone as 16623-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 16623-1-AP (ACADS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16623-1-PBS in a different storage buffer formulation.
WB analysis of COS-7 using 16623-1-AP (same clone as 16623-1-PBS)
COS-7 cells were subjected to SDS PAGE followed by western blot with 16623-1-AP (ACADS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16623-1-PBS in a different storage buffer formulation.
IP experiment of HEK-293 using 16623-1-AP (same clone as 16623-1-PBS)
IP result of anti-ACADS (IP:16623-1-AP, 4ug; Detection:16623-1-AP 1:500) with HEK-293 cells lysate 3000ug. This data was developed using the same antibody clone with 16623-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 16623-1-AP (same clone as 16623-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16623-1-AP (ACADS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16623-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 16623-1-AP (same clone as 16623-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 16623-1-AP (ACADS antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16623-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 16623-1-AP (same clone as 16623-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 16623-1-AP (ACADS antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16623-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 16623-1-AP (same clone as 16623-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16623-1-AP (ACADS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16623-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 16623-1-AP (same clone as 16623-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart using 16623-1-AP (ACADS antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16623-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 16623-1-AP (same clone as 16623-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart using 16623-1-AP (ACADS antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16623-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

ACADS Polyclonal antibody, PBS Only

Name: ACADS Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 16623-1-PBS

ACADS Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 16623-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

16623-1-PBS targets ACADS in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity	human, mouse, rat, monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9922 Product name: Recombinant human ACADS protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-412 aa of BC025963 Sequence: MAAALLARASGPARRALCPRAWRQLHTIYQSVELPETHQMLLQTCRDFAEKELFPIAAQVDKEHLFPAAQVKKMGGLGLLAMDVPEELGGAGLDYLAYAIAMEEISRGCASTGVIMSVNNSLYLGPILKFGSKEQKQAWVTPFTSGDKIGCFALSEPGNGSDAGAASTTARAEGDSWVLNGTKAWITNAWEASAAVVFASTDRALQNKSISAFLVPMPTPGLTLGKKEDKLGIRGSSTANLIFEDCRIPKDSILGEPGMGFKIAMQTLDMGRIGIASQALGIAQTALDCAVNYAENRMAFGAPLTKLQVIQFKLADMALALESARLLTWRAAMLKDNKKPFIKEAAMAKLAASEAATAISHQAIQILGGMGYVTEMPAERHYRDARITEIYEGTSEIQRLVIAGHLLRSYRS Predict reactive species
Full Name	acyl-Coenzyme A dehydrogenase, C-2 to C-3 short chain
Calculated Molecular Weight	412 aa, 44 kDa
Observed Molecular Weight	42 kDa
GenBank Accession Number	BC025963
Gene Symbol	ACADS
Gene ID (NCBI)	35
RRID	AB_10666165
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P16219
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

ACADS is also named as SCAD(Short-chain specific acyl-CoA dehydrogenase, mitochondrial) and belongs to the acyl-CoA dehydrogenase family. It is a tetrameric mitochondrial flavoprotein. It is synthesized as a 44 kDa precursor, transported into mitochondria, and proteolytically processed to its 41 kDa mature form. Acyl-CoA de- hydrogenases (ACADs) are a family of flavoproteins that are involved in theβ-oxidation of the fatty acyl-CoA derivatives.