ACAP1 Polyclonal antibody

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  WB analysis of Jurkat using 20135-1-AP

  WB result of ACAP1 antibody (20135-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACAP1 transfected Jurkat cells.

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  WB analysis using 20135-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20135-1-AP (ACAP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung using 20135-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 20135-1-AP (ACAP1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 20135-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 20135-1-AP (ACAP1 antibody) at dilution of 1:100 (under 40x lens).

• Featured Product
• KD/KO Validated

ACAP1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 20135-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

Cnt-b1, Cnt b1, CENTB1, Centaurin-beta-1, Centaurin beta 1

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Tested Applications

• Positive WB detected in: HL-60 cells, Jurkat cells
• Positive IHC detected in: human lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

20135-1-AP targets ACAP1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACAP1 fusion protein Ag14068
• Full Name: ArfGAP with coiled-coil, ankyrin repeat and PH domains 1
• Calculated Molecular Weight: 740 aa, 82 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC018543
• Gene Symbol: ACAP1
• Gene ID (NCBI): 9744
• RRID: AB_10696311
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15027
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACAP1, also named as Centaurin-beta-1, is a 740 amino acid protein, which is expressed Highly in lung and spleen. ACAP1 as a GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) is required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface and required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.

Publications

Species	Application	Title
human	WB	Comput Struct Biotechnol J; Integrated analysis and validation reveal ACAP1 as a novel prognostic biomarker associated with tumor immunity in lung adenocarcinoma; Authors - Ning Wang
human	WB,IF	Methods Cell Biol; An ACAP1 coat complex acting in endocytic recycling.; Authors - Jian Li