ACAP1 Polyclonal antibody

ACAP1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 20135-1-AP

Synonyms

ACAP1, Centaurin beta 1, CENTB1, Cnt b1, KIAA0050

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Validation Data Gallery

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  WB analysis of Jurkat using 20135-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20135-1-AP (ACAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 20135-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20135-1-AP (ACAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung using 20135-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 20135-1-AP (ACAP1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 20135-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 20135-1-AP (ACAP1 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IHC detected in: human lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

The immunogen of 20135-1-AP is ACAP1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACAP1 fusion protein Ag14068
• Full Name: ArfGAP with coiled-coil, ankyrin repeat and PH domains 1
• Calculated molecular weight: 740 aa, 82 kDa
• Observed molecular weight: 85 kDa
• GenBank accession number: BC018543
• Gene symbol: ACAP1
• Gene ID (NCBI): 9744
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Comput Struct Biotechnol J; Integrated analysis and validation reveal ACAP1 as a novel prognostic biomarker associated with tumor immunity in lung adenocarcinoma; Authors - Ning Wang
human	WB,IF	Methods Cell Biol; An ACAP1 coat complex acting in endocytic recycling.; Authors - Jian Li