ACAP1 Polyclonal antibody

ACAP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 20135-1-AP


ACAP1, Centaurin beta 1, CENTB1, Cnt b1, KIAA0050

Tested Applications

Positive WB detected inJurkat cells
Positive IHC detected inhuman lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20135-1-AP targets ACAP1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACAP1 fusion protein Ag14068
Full Name ArfGAP with coiled-coil, ankyrin repeat and PH domains 1
Calculated Molecular Weight 740 aa, 82 kDa
Observed Molecular Weight 85 kDa
GenBank Accession NumberBC018543
Gene Symbol ACAP1
Gene ID (NCBI) 9744
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACAP1, also named as Centaurin-beta-1, is a 740 amino acid protein, which is expressed Highly in lung and spleen. ACAP1 as a GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) is required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface and required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.


Product Specific Protocols
WB protocol for ACAP1 antibody 20135-1-APDownload protocol
IHC protocol for ACAP1 antibody 20135-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Comput Struct Biotechnol J

Integrated analysis and validation reveal ACAP1 as a novel prognostic biomarker associated with tumor immunity in lung adenocarcinoma

Authors - Ning Wang

Methods Cell Biol

An ACAP1 coat complex acting in endocytic recycling.

Authors - Jian Li