ACAP1 Monoclonal antibody

ACAP1 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1


Human and More (1)







Cat no : 66596-1-Ig


ACAP1, Centaurin beta 1, CENTB1, Cnt b1, KIAA0050

Tested Applications

Positive WB detected inJurkat cells, HL-60 cells, K-562 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

66596-1-Ig targets ACAP1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ACAP1 fusion protein Ag14254
Full Name ArfGAP with coiled-coil, ankyrin repeat and PH domains 1
Calculated Molecular Weight 740 aa, 82 kDa
Observed Molecular Weight 81 kDa
GenBank Accession NumberBC018543
Gene Symbol ACAP1
Gene ID (NCBI) 9744
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACAP1, also named as Centaurin-beta-1, is a 740 amino acid protein, which is expressed Highly in lung and spleen. ACAP1 as a GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) is required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface and required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.


Product Specific Protocols
WB protocol for ACAP1 antibody 66596-1-IgDownload protocol
IHC protocol for ACAP1 antibody 66596-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cancers (Basel)

ACAP1 Deficiency Predicts Inferior Immunotherapy Response in Solid Tumors

Authors - Qiyi Yi