ACAP1 Monoclonal antibody

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  WB analysis of Jurkat using 66596-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66596-1-Ig (ACAP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 66596-1-Ig

  WB result of ACAP1 antibody (66596-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACAP1 transfected Jurkat cells.

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  WB analysis of HL-60 using 66596-1-Ig

  HL-60 cells were subjected to SDS PAGE followed by western blot with 66596-1-Ig (ACAP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66596-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66596-1-Ig (ACAP1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

ACAP1 Monoclonal Antibody for WB, IHC, ELISA

Cat No. 66596-1-Ig

Clone No.1B4C9

Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Cnt b1, CENTB1, Centaurin-beta-1, Centaurin beta 1, Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1

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Tested Applications

• Positive WB detected in: Jurkat cells, HL-60 cells, K-562 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

66596-1-Ig targets ACAP1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14254

  Product name: Recombinant human ACAP1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 20-251 aa of BC018543

  Sequence: SIELVEAEVSELETRLEKLLKLGTGLLESGRHYLAASRAFVVGICDLARLGPPEPMMAECLEKFTVSLNHKLDSHAELLDATQHTLQQQIQTLVKEGLRGFREARRDFWRGAESLEAALTHNAEVPRRRAQEAEEAGAALRTARAGYRGRALDYALQINVIEDKRKFDIMEFVLRLVEAQATHFQQGHEELSRLSQYRKELGAQLHQLVLNSAREKRDMEQRHVLLKQKELG

• Full Name: ArfGAP with coiled-coil, ankyrin repeat and PH domains 1
• Calculated Molecular Weight: 740 aa, 82 kDa
• Observed Molecular Weight: 81 kDa
• GenBank Accession Number: BC018543
• Gene Symbol: ACAP1
• Gene ID (NCBI): 9744
• RRID: AB_2881956
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q15027
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACAP1, also named as Centaurin-beta-1, is a 740 amino acid protein, which is expressed Highly in lung and spleen. ACAP1 as a GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) is required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface and required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cancers (Basel); ACAP1 Deficiency Predicts Inferior Immunotherapy Response in Solid Tumors; Authors - Qiyi Yi