ACAP1 Monoclonal antibody
ACAP1 Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (1)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
1B4C9
Cat no : 66596-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells, HL-60 cells, K-562 cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 1 publications below |
Product Information
66596-1-Ig targets ACAP1 in WB, IHC, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Cited Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | ACAP1 fusion protein Ag14254 |
Full Name | ArfGAP with coiled-coil, ankyrin repeat and PH domains 1 |
Calculated Molecular Weight | 740 aa, 82 kDa |
Observed Molecular Weight | 81 kDa |
GenBank Accession Number | BC018543 |
Gene Symbol | ACAP1 |
Gene ID (NCBI) | 9744 |
RRID | AB_2881956 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ACAP1, also named as Centaurin-beta-1, is a 740 amino acid protein, which is expressed Highly in lung and spleen. ACAP1 as a GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) is required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface and required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for ACAP1 antibody 66596-1-Ig | Download protocol |
IHC protocol for ACAP1 antibody 66596-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cancers (Basel) ACAP1 Deficiency Predicts Inferior Immunotherapy Response in Solid Tumors |