ACAP1 Monoclonal antibody, PBS Only

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  WB analysis of Jurkat using 66596-1-Ig (same clone as 66596-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66596-1-Ig (ACAP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66596-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66596-1-Ig (same clone as 66596-1-PBS)

  WB result of ACAP1 antibody (66596-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACAP1 transfected Jurkat cells. This data was developed using the same antibody clone with 66596-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HL-60 using 66596-1-Ig (same clone as 66596-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 66596-1-Ig (ACAP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66596-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66596-1-Ig (same clone as 66596-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66596-1-Ig (ACAP1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66596-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

ACAP1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 66596-1-PBS

Clone No.1B4C9

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Cnt b1, CENTB1, Centaurin-beta-1, Centaurin beta 1, Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1

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Product Information

66596-1-PBS targets ACAP1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14254

  Product name: Recombinant human ACAP1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 20-251 aa of BC018543

  Sequence: SIELVEAEVSELETRLEKLLKLGTGLLESGRHYLAASRAFVVGICDLARLGPPEPMMAECLEKFTVSLNHKLDSHAELLDATQHTLQQQIQTLVKEGLRGFREARRDFWRGAESLEAALTHNAEVPRRRAQEAEEAGAALRTARAGYRGRALDYALQINVIEDKRKFDIMEFVLRLVEAQATHFQQGHEELSRLSQYRKELGAQLHQLVLNSAREKRDMEQRHVLLKQKELG

• Full Name: ArfGAP with coiled-coil, ankyrin repeat and PH domains 1
• Calculated Molecular Weight: 740 aa, 82 kDa
• Observed Molecular Weight: 81 kDa
• GenBank Accession Number: BC018543
• Gene Symbol: ACAP1
• Gene ID (NCBI): 9744
• RRID: AB_2881956
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q15027
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ACAP1, also named as Centaurin-beta-1, is a 740 amino acid protein, which is expressed Highly in lung and spleen. ACAP1 as a GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) is required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface and required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.