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ACAT1 Polyclonal antibody

ACAT1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16215-1-AP

Synonyms

ACAT, ACAT1, Acetoacetyl CoA thiolase, MAT, T2, THIL



Tested Applications

Positive WB detected inmouse heart tissue, rat skeletal muscle tissue, human skeletal muscle tissue, MCF-7 cells
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

16215-1-AP targets ACAT1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACAT1 fusion protein Ag9215
Full Name acetyl-Coenzyme A acetyltransferase 1
Calculated molecular weight 45 kDa
Observed molecular weight 38-45 kDa
GenBank accession numberBC010942
Gene symbol ACAT1
Gene ID (NCBI) 38
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACAT1 and ACAT2 are intracellular enzymes that convert free cholesterol to cholesteryl esters and play important roles in lipid metabolism. ACAT1 is widely expressed while ACAT2 is primarily expressed in intestine and liver. As the major ACAT isozyme in macrophages, ACTA1 plays an key role in foam cell formation and atherosclerosis, making it an appealing drug target for treating cardiovascular diseases. Recently it has been reported that ACTA1 is commonly activated by upregulated tetramerization in human cancers and is a promising anti-cancer target.

Protocols

Product Specific Protocols
WB protocol for ACAT1 antibody 16215-1-APDownload protocol
IHC protocol for ACAT1 antibody 16215-1-APDownload protocol
IF protocol for ACAT1 antibody 16215-1-APDownload protocol
IP protocol for ACAT1 antibody 16215-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Genes Dev

TGIF transcription factors repress acetyl CoA metabolic gene expression and promote intestinal tumor growth.

Authors - Anant Shah
humanWB,IHC

Front Oncol

ACAT1 and Metabolism-Related Pathways Are Essential for the Progression of Clear Cell Renal Cell Carcinoma (ccRCC), as Determined by Co-expression Network Analysis.

Authors - Liang Chen
humanWB

Biomolecules

Carbamoyl-Phosphate Synthase 1 as a Novel Target of Phomoxanthone A, a Bioactive Fungal Metabolite.

Authors - Sara Ceccacci
humanWB

Nat Metab

Myc-mediated SDHA acetylation triggers epigenetic regulation of gene expression and tumorigenesis.

Authors - Shi-Ting Li
humanWB

Arch Med Sci

Effects of Porphyromonas gingivalis lipopolysaccharide on the expression of key genes involved in cholesterol metabolism in macrophages.

Authors - Fen Liu
humanWB

Molecules

Mitochondrion-Targeted Peptide SS-31 Inhibited Oxidized Low-Density Lipoproteins-Induced Foam Cell Formation through both ROS Scavenging and Inhibition of Cholesterol Influx in RAW264.7 Cells.

Authors - Shuangying Hao