ACAT1 Polyclonal antibody

• 

  WB analysis of MCF-7 using 16215-1-AP

  WB result of ACAT1 antibody (16215-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACAT1 transfected MCF-7 cells.

• 

  WB analysis using 16215-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16215-1-AP (ACAT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 16215-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16215-1-AP (ACAT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 16215-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16215-1-AP (ACAT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skeletal muscle using 16215-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16215-1-AP (ACAT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 16215-1-AP

  IP result of anti-ACAT1 (IP:16215-1-AP, 4ug; Detection:16215-1-AP 1:500) with mouse heart tissue lysate 4400ug.

• 

  IHC staining of human liver using 16215-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16215-1-AP (ACAT1 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 16215-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16215-1-AP (ACAT1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 16215-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16215-1-AP (ACAT1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human colon using 16215-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 16215-1-AP (ACAT1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 16215-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ACAT1 antibody (16215-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  FC experiment of HeLa using 16215-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ACAT1 (16215-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

ACAT1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 16215-1-AP

Publications(37)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

ACAT, Acetoacetyl CoA thiolase, Acetoacetyl-CoA thiolase, Acetyl-CoA acetyltransferase, mitochondrial, EC:2.3.1.9

---

Tested Applications

• Positive WB detected in: mouse heart tissue, Caco-2 cells, human skeletal muscle tissue, MCF-7 cells, rat skeletal muscle tissue, mouse colon tissue
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human colon, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 32 publications below
• IHC: See 6 publications below
• IF: See 5 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

16215-1-AP targets ACAT1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, rabbit
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACAT1 fusion protein Ag9215
• Full Name: acetyl-Coenzyme A acetyltransferase 1
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 38-45 kDa
• GenBank Accession Number: BC010942
• Gene Symbol: ACAT1
• Gene ID (NCBI): 38
• RRID: AB_2220210
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P24752
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACAT1 (acetyl-CoA acetyltransferase), is a mitochondrial enzyme catalyzing the reversible formation or breakdown of acetoacetyl-CoA and plays important roles in ketogenesis and ketolysis. Deficiency of ACAT1 causes an inherited metabolic disorder. Recently ACAT1 has been reported to get involved in cancer progression.

Publications

Species	Application	Title
human	IF	Adv Mater; Supramolecular Hydrogel with Ultra-Rapid Cell-Mediated Network Adaptation for Enhancing Cellular Metabolic Energetics and Tissue Regeneration; Authors - Zhuo Li
mouse	WB	Cell Metab; TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.; Authors - Dong Huang
human	WB	Cell Res; Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress.; Authors - De Huang
Mouse	WB	J Clin Invest; Acetaldehyde dehydrogenase 2 interactions with LDLR and AMPK regulate foam cell formation.; Authors - Shanshan Zhong
mouse	WB	Sci Adv; Ketogenic diet uncovers differential metabolic plasticity of brain cells; Authors - Tim Düking
mouse,human	WB	Phytomedicine; Rice bran active peptide (RBAP) inhibited macrophage differentiation to foam cell and atherosclerosis in mice via regulating cholesterol efflux; Authors - Jianfei Mu