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- KD/KO Validated
ACAT1 Polyclonal antibody
ACAT1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IP, IHC, IF, CoIP, ELISA
Cat no : 16215-1-AP
Validation Data Gallery
|Positive WB detected in||mouse heart tissue, rat skeletal muscle tissue, human skeletal muscle tissue, MCF-7 cells|
|Positive IP detected in||mouse heart tissue|
|Positive IHC detected in||human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Hela cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 1 publications below|
|WB||See 17 publications below|
|IHC||See 4 publications below|
|IF||See 3 publications below|
|IP||See 1 publications below|
|CoIP||See 1 publications below|
16215-1-AP targets ACAT1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ACAT1 fusion protein Ag9215|
|Full Name||acetyl-Coenzyme A acetyltransferase 1|
|Calculated molecular weight||45 kDa|
|Observed molecular weight||38-45 kDa|
|GenBank accession number||BC010942|
|Gene ID (NCBI)||38|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
ACAT1 (acetyl-CoA acetyltransferase), is a mitochondrial enzyme catalyzing the reversible formation or breakdown of acetoacetyl-CoA and plays important roles in ketogenesis and ketolysis. Deficiency of ACAT1 causes an inherited metabolic disorder. Recently ACAT1 has been reported to get involved in cancer progression.
|Product Specific Protocols|
|WB protocol for ACAT1 antibody 16215-1-AP||Download protocol|
|IHC protocol for ACAT1 antibody 16215-1-AP||Download protocol|
|IF protocol for ACAT1 antibody 16215-1-AP||Download protocol|
|IP protocol for ACAT1 antibody 16215-1-AP||Download protocol|
|Click here to view our Standard Protocols|
TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.
Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress.
Ketogenic diet uncovers differential metabolic plasticity of brain cells
Hum Mol Genet
Partial Complex I deficiency due to the CNS conditional ablation of Ndufa5 results in a mild chronic encephalopathy but no increase in oxidative damage.
Cell Mol Gastroenterol Hepatol
S100A11 Promotes Liver Steatosis via FOXO1-Mediated Autophagy and Lipogenesis.
Ketone body β-hydroxybutyrate ameliorates colitis by promoting M2 macrophage polarization through the STAT6-dependent signaling pathway.