ACAT1 Polyclonal antibody

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  WB analysis of MCF-7 using 16215-1-AP

  WB result of ACAT1 antibody (16215-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACAT1 transfected MCF-7 cells.

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  WB analysis using 16215-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16215-1-AP (ACAT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 16215-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16215-1-AP (ACAT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 16215-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16215-1-AP (ACAT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat skeletal muscle using 16215-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16215-1-AP (ACAT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 16215-1-AP

  IP result of anti-ACAT1 (IP:16215-1-AP, 4ug; Detection:16215-1-AP 1:500) with mouse heart tissue lysate 4400ug.

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  IHC staining of human liver using 16215-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16215-1-AP (ACAT1 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 16215-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16215-1-AP (ACAT1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 16215-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16215-1-AP (ACAT1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human colon using 16215-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 16215-1-AP (ACAT1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 16215-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ACAT1 antibody (16215-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HeLa using 16215-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ACAT1 (16215-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

ACAT1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 16215-1-AP

Publications(37)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

ACAT, Acetoacetyl CoA thiolase, Acetoacetyl-CoA thiolase, Acetyl-CoA acetyltransferase, mitochondrial, EC:2.3.1.9

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Tested Applications

• Positive WB detected in: mouse heart tissue, Caco-2 cells, human skeletal muscle tissue, MCF-7 cells, rat skeletal muscle tissue, mouse colon tissue
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human colon, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 32 publications below
• IHC: See 6 publications below
• IF: See 5 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

16215-1-AP targets ACAT1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, rabbit
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACAT1 fusion protein Ag9215
• Full Name: acetyl-Coenzyme A acetyltransferase 1
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 38-45 kDa
• GenBank Accession Number: BC010942
• Gene Symbol: ACAT1
• Gene ID (NCBI): 38
• RRID: AB_2220210
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P24752
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACAT1 (acetyl-CoA acetyltransferase), is a mitochondrial enzyme catalyzing the reversible formation or breakdown of acetoacetyl-CoA and plays important roles in ketogenesis and ketolysis. Deficiency of ACAT1 causes an inherited metabolic disorder. Recently ACAT1 has been reported to get involved in cancer progression.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Adv Mater; Supramolecular Hydrogel with Ultra-Rapid Cell-Mediated Network Adaptation for Enhancing Cellular Metabolic Energetics and Tissue Regeneration; Authors - Zhuo Li
mouse	WB	Cell Metab; TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.; Authors - Dong Huang
human	WB	Cell Res; Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress.; Authors - De Huang
Mouse	WB	J Clin Invest; Acetaldehyde dehydrogenase 2 interactions with LDLR and AMPK regulate foam cell formation.; Authors - Shanshan Zhong
mouse	WB	Sci Adv; Ketogenic diet uncovers differential metabolic plasticity of brain cells; Authors - Tim Düking
mouse,human	WB	Phytomedicine; Rice bran active peptide (RBAP) inhibited macrophage differentiation to foam cell and atherosclerosis in mice via regulating cholesterol efflux; Authors - Jianfei Mu