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ACAT1 Antibody 9 Publications

Rabbit Polyclonal| Catalog number: 16215-1-AP

Featured Product KD/KO validated

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Con: 30 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

ACAT1 Antibody WB MCF-7 cells 16215-1-AP

WB result of ACAT1 antibody (16215-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACAT1 transfected MCF-7 cells

ACAT1 Antibody WB Caco-2 cells 16215-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 16215-1-AP (ACAT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

ACAT1 Antibody IHC human liver tissue 16215-1-AP

Immunohistochemical analysis of paraffin-embedded human liver using 16215-1-AP(ACAT1 antibody) at dilution of 1:100 (under 10x lens)

ACAT1 Antibody IHC human liver tissue 16215-1-AP

Immunohistochemical analysis of paraffin-embedded human liver using 16215-1-AP(ACAT1 antibody) at dilution of 1:100 (under 40x lens)

ACAT1 Antibody IF Hela cells 16215-1-AP

Immunofluorescent analysis of Hela cells, using ACAT1 antibody 16215-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

ACAT1 Antibody IP mouse heart tissue 16215-1-AP

IP Result of anti-ACAT1 (IP:16215-1-AP, 4ug; Detection:16215-1-AP 1:500) with mouse heart tissue lysate 4400ug.

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse heart tissue, rat skeletal muscle tissue, human skeletal muscle tissue, MCF-7 cells
Positive IP detected in:
mouse heart tissue
Positive IHC detected in:
human liver tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
Hela cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:20-1:200
IF : 1:10-1:100
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ACAT1 fusion protein Ag9215
Full name:
acetyl-Coenzyme A acetyltransferase 1
Calculated molecular weight:
45 kDa
Observed molecular weight:
38-45 kDa
GenBank accession number:
BC010942
Gene ID (NCBI):
38
Gene symbol
ACAT1
Synonyms
ACAT, ACAT1, Acetoacetyl CoA thiolase, MAT, T2, THIL

Background

ACAT1 and ACTA2 are intracellular enzymes that convert free cholesterol to cholesteryl esters and play important roles in lipid metabolism. ACAT1 is widely expressed while ACTA2 is primarily expressed in intestine and liver. As the major ACAT isozyme in macrophages, ACTA1 plays an key role in foam cell formation and atherosclerosis, making it an appealing drug target for treating cardiovascular diseases. Recently it has been reported that ACTA1 is commonly activated by upregulated tetramerization in human cancers and is a promising anti-cancer target.


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