Tested Applications
| Positive WB detected in | mouse heart tissue, Caco-2 cells, human skeletal muscle tissue, MCF-7 cells, rat skeletal muscle tissue, mouse colon tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human colon, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16215-1-AP targets ACAT1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, IP-MS, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, rabbit |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9215 Product name: Recombinant human ACAT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-162 aa of BC010942 Sequence: MAVLPALLRSGARSRSPLLRRLVQEIRYVERSYVSKPTLKEVVIVSATRTPIGSFLGSLSLLPATKLGSIAIQGAIEKAGIPKEEVKEAYMGNVLQGGEGQAPTRQAVLGAGLPISTPCTTINKVCASGMKAIMMASQSLMCGHQIKQETGSLAKICCHVRR Predict reactive species |
| Full Name | acetyl-Coenzyme A acetyltransferase 1 |
| Calculated Molecular Weight | 45 kDa |
| Observed Molecular Weight | 38-45 kDa |
| GenBank Accession Number | BC010942 |
| Gene Symbol | ACAT1 |
| Gene ID (NCBI) | 38 |
| RRID | AB_2220210 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P24752 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ACAT1 (acetyl-CoA acetyltransferase), is a mitochondrial enzyme catalyzing the reversible formation or breakdown of acetoacetyl-CoA and plays important roles in ketogenesis and ketolysis. Deficiency of ACAT1 causes an inherited metabolic disorder. Recently ACAT1 has been reported to get involved in cancer progression.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for ACAT1 antibody 16215-1-AP | Download protocol |
| IF protocol for ACAT1 antibody 16215-1-AP | Download protocol |
| IHC protocol for ACAT1 antibody 16215-1-AP | Download protocol |
| IP protocol for ACAT1 antibody 16215-1-AP | Download protocol |
| WB protocol for ACAT1 antibody 16215-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ACAT1 Polyclonal antibody (Cat# 16215-1-AP) has 59 citations. Key journals include Nature Communications, Nature Metabolism, Nature Neuroscience, Cell Metabolism, Cell Research, Nature Chemical Biology, Neuron, Journal of Clinical Investigation, and Science Advances. Research fields span lipid metabolism, fatty acid oxidation, ketone body biology, cancer (hepatocellular, renal, prostate, gastric, ovarian, lung), cardiovascular disease, atherosclerosis, neuroscience, liver fibrosis, and epigenetic regulation. Applications are predominantly Western blot, with IF, IHC, IP, and Co-IP also represented.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther A two-strata energy flux system driven by a stress hormone prioritizes cardiac energetics | ||
Cell Metab TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis. | ||
Cell Res Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress. | ||
Adv Mater Supramolecular Hydrogel with Ultra-Rapid Cell-Mediated Network Adaptation for Enhancing Cellular Metabolic Energetics and Tissue Regeneration | ||
Nat Metab Myc-mediated SDHA acetylation triggers epigenetic regulation of gene expression and tumorigenesis. | ||
Nat Neurosci Oligodendroglial fatty acid metabolism as a central nervous system energy reserve |
