ACAT1 Polyclonal antibody, PBS Only

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  WB analysis of MCF-7 using 16215-1-AP (same clone as 16215-1-PBS)

  WB result of ACAT1 antibody (16215-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACAT1 transfected MCF-7 cells. This data was developed using the same antibody clone with 16215-1-PBS in a different storage buffer formulation.

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  WB analysis using 16215-1-AP (same clone as 16215-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16215-1-AP (ACAT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16215-1-PBS in a different storage buffer formulation.

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  WB analysis of human skeletal muscle using 16215-1-AP (same clone as 16215-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16215-1-AP (ACAT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16215-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse heart using 16215-1-AP (same clone as 16215-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16215-1-AP (ACAT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16215-1-PBS in a different storage buffer formulation.

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  WB analysis of rat skeletal muscle using 16215-1-AP (same clone as 16215-1-PBS)

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16215-1-AP (ACAT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16215-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse heart using 16215-1-AP (same clone as 16215-1-PBS)

  IP result of anti-ACAT1 (IP:16215-1-AP, 4ug; Detection:16215-1-AP 1:500) with mouse heart tissue lysate 4400ug. This data was developed using the same antibody clone with 16215-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 16215-1-AP (same clone as 16215-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16215-1-AP (ACAT1 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16215-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 16215-1-AP (same clone as 16215-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 16215-1-AP (ACAT1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16215-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 16215-1-AP (same clone as 16215-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 16215-1-AP (ACAT1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16215-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 16215-1-AP (same clone as 16215-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon slide using 16215-1-AP (ACAT1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16215-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 16215-1-AP (same clone as 16215-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ACAT1 antibody (16215-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16215-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 16215-1-AP (same clone as 16215-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ACAT1 (16215-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 16215-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

ACAT1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Cat No. 16215-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

ACAT, Acetoacetyl CoA thiolase, Acetoacetyl-CoA thiolase, Acetyl-CoA acetyltransferase, mitochondrial, EC:2.3.1.9

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Product Information

16215-1-PBS targets ACAT1 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9215

  Product name: Recombinant human ACAT1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-162 aa of BC010942

  Sequence: MAVLPALLRSGARSRSPLLRRLVQEIRYVERSYVSKPTLKEVVIVSATRTPIGSFLGSLSLLPATKLGSIAIQGAIEKAGIPKEEVKEAYMGNVLQGGEGQAPTRQAVLGAGLPISTPCTTINKVCASGMKAIMMASQSLMCGHQIKQETGSLAKICCHVRR

• Full Name: acetyl-Coenzyme A acetyltransferase 1
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 38-45 kDa
• GenBank Accession Number: BC010942
• Gene Symbol: ACAT1
• Gene ID (NCBI): 38
• RRID: AB_2220210
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P24752
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ACAT1 (acetyl-CoA acetyltransferase), is a mitochondrial enzyme catalyzing the reversible formation or breakdown of acetoacetyl-CoA and plays important roles in ketogenesis and ketolysis. Deficiency of ACAT1 causes an inherited metabolic disorder. Recently ACAT1 has been reported to get involved in cancer progression.