ACAT2 Polyclonal antibody

ACAT2 Polyclonal Antibody for FC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, FC

Conjugate

Unconjugated

Cat no : 14755-1-AP

Synonyms

ACAT2, ACTL



Tested Applications

Positive WB detected inmouse liver tissue, L02 cells, Caco-2 cells, HepG2 cells
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver tissue, human lung tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 5 publications below

Product Information

14755-1-AP targets ACAT2 in WB, IP, IHC, FC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACAT2 fusion protein Ag6503
Full Name acetyl-Coenzyme A acetyltransferase 2
Calculated molecular weight 41 kDa
Observed molecular weight 40-42 kDa
GenBank accession numberBC000408
Gene symbol ACAT2
Gene ID (NCBI) 39
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ACAT2 antibody 14755-1-APDownload protocol
IHC protocol for ACAT2 antibody 14755-1-APDownload protocol
IP protocol for ACAT2 antibody 14755-1-APDownload protocol
FC protocol for ACAT2 antibody 14755-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Cell Res

Blocking FSH inhibits hepatic cholesterol biosynthesis and reduces serum cholesterol.

Authors - Yanjing Guo
humanWB

Cell Res

Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress.

Authors - De Huang
mouseWB

iScience

Hepatocyte activity of the cholesterol sensor smoothened regulates cholesterol and bile acid homeostasis in mice.

Authors - George D Dalton
mouseWB

J Agric Food Chem

Oral Administration of Succinoglycan Riclin Improves Diet-Induced Hypercholesterolemia in Mice.

Authors - Yunxia Yang
humanWB

Food Res Int

Wild Lonicera caerulea berry polyphenol extract reduces cholesterol accumulation and enhances antioxidant capacity in vitro and in vivo.

Authors - Suwen Liu
ratWB

Biochem Biophys Res Commun

Exosomal and extracellular HMGB1 have opposite effects on SASH1 expression in rat astrocytes and glioma C6 cells.

Authors - Chao Ma