ACO2 Antibody

ACO2 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1F1G4

Cat no : 67509-1-Ig

Synonyms

ACO2, Aconitase, aconitase 2, mitochondrial, ACONM, Citrate hydro lyase



Tested Applications

Positive WB detected inpig brain tissue, mouse brain tissue, rat brain tissue, pig cerebellum tissue, rat cerebellum tissue, mouse cerebellum tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

67509-1-Ig targets ACO2 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ACO2 fusion protein Ag17784
Full Name aconitase 2, mitochondrial
Calculated molecular weight 85 kDa
Observed molecular weight 85 kDa
GenBank accession number BC014092
Gene symbol ACO2
Gene ID (NCBI) 50
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

ACO2(aconitate hydratase, mitochondrial) is also named as citrate hydro-lyase and belongs to the aconitase/IPM isomerase family. It plays a key function in cellular energy production, and loss of its activity has a major impact on cellular and organismal survival. Western blot shows two bands of 83 kDa and 40 kDa. The 40 kDa fragment decreases with age and oxidative stress, whereas other fragmentation products with molecular weights between 40 and 83 kDa increased with age and MnSOD(mitochondrial manganese superoxide dismutase) deficiency(PMID:12459471). Defects in ACO2 are the cause of infantile cerebellar-retinal degeneration (ICRD).

Protocols

Product Specific Protocols
WB protocol for ACO2 antibody 67509-1-IgDownload protocol
IHC protocol for ACO2 antibody 67509-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols