ACOT9 Antibody

ACOT9 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3F10G5

Cat no : 66532-1-Ig

Synonyms

ACATE2, ACOT9, Acyl CoA thioester hydrolase 9, acyl CoA thioesterase 9, CGI 16, MT ACT48



Tested Applications

Positive WB detected inHEK-293 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66532-1-Ig targets ACOT9 in WB, IHC, ELISA applications and shows reactivity with Human, Rat samples.

Tested Reactivity Human, Rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ACOT9 fusion protein Ag8884
Full Name acyl-CoA thioesterase 9
Calculated molecular weight 24 kDa;49 kDa
Observed molecular weight 43-50 kDa
GenBank accession number BC012573
Gene symbol ACOT9
Gene ID (NCBI) 23597
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACOT9 (Acyl-coenzyme A thioesterase 9) is a member of the acyl-CoA thioesterase superfamily, which is a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. ACOT9 has 4 isoforms with the molecular mass of 43-50 kDa.

Protocols

Product Specific Protocols
WB protocol for ACOT9 antibody 66532-1-IgDownload protocol
IHC protocol for ACOT9 antibody 66532-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols