ACOX3 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 84027-4-RR (same clone as 84027-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84027-4-RR (ACOX3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84027-4-PBS in a different storage buffer formulation.

• 

  FC experiment of A431 using 84027-4-RR (same clone as 84027-4-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug ACOX3 Recombinant antibody (84027-4-RR, Clone:241172G8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 84027-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84027-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84027-4-RR against Human ACOX3 were performed. The affinity constant is 1.05 nM.

ACOX3 Uni-rAb^® Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Cat No. 84027-4-PBS

Clone No.241172G8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

241172G8, ACOX 3, Branched-chain acyl-CoA oxidase, BRCACox, BRCOX

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Product Information

84027-4-PBS targets ACOX3 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag10553

  Product name: Recombinant human ACOX3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 332-624 aa of BC017053

  Sequence: TRRQFGPTEEEEIPVLEYPMQQWRLLPYLAAVYALDHFSKSLFLDLVELQRGLASGDRSARQAELGREIHALASASKPLASWTTQQGIQECREACGGHGYLAMNRLGVLRDDNDPNCTYEGDNNILLQQTSNYLLGLLAHQVHDGACFRSPLKSVDFLDAYPGILDQKFEVSSVADCLDSAVALAAYKWLVCYLLRETYQKLNQEKRSGSSDFEARNKCQVSHGRPLALAFVELTVVQRFHEHVHQPSVPPSLRAVLGRLSALYALWSLSRHAALLYRAERRCSCPGRRDRSS

• Full Name: acyl-Coenzyme A oxidase 3, pristanoyl
• Calculated Molecular Weight: 624 aa, 70 kDa
• Observed Molecular Weight: 70-78 kDa
• GenBank Accession Number: BC017053
• Gene Symbol: ACOX3
• Gene ID (NCBI): 8310
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O15254
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Acyl-Coenzyme A oxidase 3 also know as pristanoyl -CoA oxidase (ACOX3) is involved in the desaturation of 2-methyl branched fatty acids in peroxisomes.