Filter:
Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, MCF-7 cells, HL-60 cells, U-251 cells, A549 cells，human placenta tissue |
| Positive FC (Intra) detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84027-4-RR targets ACOX3 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag10553 Product name: Recombinant human ACOX3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 332-624 aa of BC017053 Sequence: TRRQFGPTEEEEIPVLEYPMQQWRLLPYLAAVYALDHFSKSLFLDLVELQRGLASGDRSARQAELGREIHALASASKPLASWTTQQGIQECREACGGHGYLAMNRLGVLRDDNDPNCTYEGDNNILLQQTSNYLLGLLAHQVHDGACFRSPLKSVDFLDAYPGILDQKFEVSSVADCLDSAVALAAYKWLVCYLLRETYQKLNQEKRSGSSDFEARNKCQVSHGRPLALAFVELTVVQRFHEHVHQPSVPPSLRAVLGRLSALYALWSLSRHAALLYRAERRCSCPGRRDRSS Predict reactive species |
| Full Name | acyl-Coenzyme A oxidase 3, pristanoyl |
| Calculated Molecular Weight | 624 aa, 70 kDa |
| Observed Molecular Weight | 70-78 kDa |
| GenBank Accession Number | BC017053 |
| Gene Symbol | ACOX3 |
| Gene ID (NCBI) | 8310 |
| RRID | AB_3671594 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | O15254 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Acyl-Coenzyme A oxidase 3 also know as pristanoyl -CoA oxidase (ACOX3) is involved in the desaturation of 2-methyl branched fatty acids in peroxisomes.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for ACOX3 antibody 84027-4-RR | Download protocol |
| WB protocol for ACOX3 antibody 84027-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
