ACSM5 Antibody

ACSM5 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2D5D6

Cat no : 67334-1-Ig

Synonyms

ACSM5, FLJ20581, MACS3



Tested Applications

Positive WB detected in4T1 cells, MDA-MB-231 cells, rat liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

67334-1-Ig targets ACSM5 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ACSM5 fusion protein Ag10056
Full Name acyl-CoA synthetase medium-chain family member 5
Calculated molecular weight 208aa,23 kDa
Observed molecular weight 60-65 kDa
GenBank accession number BC016703
Gene symbol ACSM5
Gene ID (NCBI) 54988
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

ACSM5(Acyl-CoA synthetase medium-chain family member 5) is also named as MACS3 and belongs to the ATP-dependent AMP-binding enzyme family. ACSM5 has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). This protein has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for ACSM5 antibody 67334-1-IgDownload protocol
IHC protocol for ACSM5 antibody 67334-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols