Filter: AllWBIHCIF/ICC

WB analysis of 4T1 using 67334-1-Ig (same clone as 67334-1-PBS)
4T1 cells were subjected to SDS PAGE followed by western blot with 67334-1-Ig (ACSM5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67334-1-PBS in a different storage buffer formulation.
WB analysis of rat liver using 67334-1-Ig (same clone as 67334-1-PBS)
rat liver tissue were subjected to SDS PAGE followed by western blot with 67334-1-Ig (ACSM5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67334-1-PBS in a different storage buffer formulation.
WB analysis of MDA-MB-231 using 67334-1-Ig (same clone as 67334-1-PBS)
MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 67334-1-Ig (ACSM5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67334-1-PBS in a different storage buffer formulation.
WB analysis of 4T1 using 67334-1-Ig (same clone as 67334-1-PBS)
4T1 cells were subjected to SDS PAGE followed by western blot with 67334-1-Ig (ACSM5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67334-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 67334-1-Ig (same clone as 67334-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67334-1-Ig (ACSM5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67334-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 67334-1-Ig (same clone as 67334-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67334-1-Ig (ACSM5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67334-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 67334-1-Ig (same clone as 67334-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67334-1-Ig (ACSM5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67334-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 67334-1-Ig (same clone as 67334-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67334-1-Ig (ACSM5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67334-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 67334-1-Ig (same clone as 67334-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ACSM5 antibody (67334-1-Ig, Clone: 2D5D6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 67334-1-PBS in a different storage buffer formulation.

ACSM5 Monoclonal antibody, PBS Only

ACSM5 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 67334-1-PBS

Clone No.2D5D6

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

2D5D6, ACSM 5, Acyl-CoA synthetase medium-chain family member 5, Acyl-coenzyme A synthetase ACSM5, mitochondrial, EC:6.2.1.2

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

67334-1-PBS targets ACSM5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag10056 Product name: Recombinant human ACSM5 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-208 aa of BC016703 Sequence: MRPWLRHLVLQALRNSRAFCGSHGKPAPLPVPQKIVATWEAISLGRQLVPEYFNFAHDVLDVWSRLEEAGHRPPNPAFWWVNGTGAEIKWSFEELGKQSRKAANVLGGACGLQPGDRMMLVLPRLPEWWLVSVACMRTGTVVIPGVTQLTEKDLKYRLQASRAKSIITSDSLAPRVDAISAECPSLQTKLLVSDSSRPGWLNFRELLR Predict reactive species
Full Name	acyl-CoA synthetase medium-chain family member 5
Calculated Molecular Weight	208 aa, 23 kDa
Observed Molecular Weight	60-65 kDa
GenBank Accession Number	BC016703
Gene Symbol	ACSM5
Gene ID (NCBI)	54988
RRID	AB_2882592
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	Q6NUN0
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

ACSM5(Acyl-CoA synthetase medium-chain family member 5) is also named as MACS3 and belongs to the ATP-dependent AMP-binding enzyme family. ACSM5 has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). This protein has 2 isoforms produced by alternative splicing.