ACTA1-Specific Polyclonal antibody

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  WB analysis of human skeletal muscle using 17521-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17521-1-AP (ACTA1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 17521-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17521-1-AP (ACTA1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat skeletal muscle using 17521-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17521-1-AP (ACTA1-Specific Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human skeletal muscle using 17521-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17521-1-AP (ACTA1-Specific Antibody) at dilution of 1:500 (under 10x lens).

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  IHC staining of human skeletal muscle using 17521-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17521-1-AP (ACTA1-Specific Antibody) at dilution of 1:500 (under 40x lens).

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  IHC staining of human hysteromyoma using 17521-1-AP

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma using 17521-1-AP (ACTA1-Specific antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human hysteromyoma using 17521-1-AP

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma using 17521-1-AP (ACTA1-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 17521-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 17521-1-AP (ACTA1-Specific Antibody) at dilution of 1:50.

• 

  IHC staining of human skeletal muscle using 17521-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 17521-1-AP (ACTA1-Specific Antibody) at dilution of 1:50.

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  FC experiment of C2C12 using 17521-1-AP

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human ACTA1-Specific (17521-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

ACTA1-Specific Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Cat No. 17521-1-AP

Publications(12)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC (Intra), ELISA

Synonyms

ACTA1, Alpha Actin, α actin, ACTA, Actin, alpha skeletal muscle, intermediate form

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Tested Applications

• Positive WB detected in: human skeletal muscle tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue
• Positive IHC detected in: human skeletal muscle tissue, human hysteromyoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: C2C12 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Product Information

17521-1-AP targets ACTA1-Specific in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: actin, alpha 1, skeletal muscle
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 39-42 kDa
• GenBank Accession Number: NM_001100
• Gene Symbol: ACTA1
• Gene ID (NCBI): 58
• RRID: AB_2273608
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P68133
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTA1 gene encodes skeletal muscle alpha-actin, the principal actin isoform in adult skeletal muscle, which interacts with a variety of proteins to produce the force for muscle contraction. There is only the N-termninal 8 amino acids difference among the actin family members. This antibody is specific to the ACTA1. It does not cross-react with other actin isoforms.

Publications

Species	Application	Title
mouse	IF	J Clin Invest; Impaired activity of the fusogenic micropeptide Myomixer causes myopathy resembling Carey-Fineman-Ziter syndrome.; Authors - Andres Ramirez-Martinez
human	WB	Cancer Lett; Cancer-associated fibroblasts-derived exosomal miR-17-5p promotes colorectal cancer aggressive phenotype by initiating a RUNX3/MYC/TGF-β1 positive feedback loop.; Authors - Yue Zhang
mouse	WB	Mol Ther Nucleic Acids; Diverging targets mediate the pathological roleof miR-199a-5p and miR-199a-3p by promoting cardiac hypertrophy and fibrosis; Authors - Ni Zeng
human	WB	Int J Mol Sci; Neddylation Regulates Class IIa and III Histone Deacetylases to Mediate Myoblast Differentiation; Authors - Hongyi Zhou
rat	IF,WB	Aging (Albany NY); Enriched environment remedies cognitive dysfunctions and synaptic plasticity through NMDAR-Ca2+-Activin A circuit in chronic cerebral hypoperfusion rats.; Authors - Xin Zhang
mouse	WB	iScience; A defective mechanosensing pathway affects fibroblast-to-myofibroblast transition in the old male mouse heart; Authors - Aude Angelini