ACTA1-Specific Polyclonal antibody

ACTA1-Specific Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 17521-1-AP

Synonyms

ACTA, ACTA1, ACTA1 skeletal Muscle Actin, ACTA1 skeletal Muscle Actin Specific, ACTA1-Specific, Actin, alpha skeletal muscle, Alpha actin 1, ASMA, CFTD, CFTD1, CFTDM, MPFD, NEM1, NEM2, NEM3, sarcomeric actin

---

Validation Data Gallery

• 

  WB analysis of human skeletal muscle using 17521-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17521-1-AP (ACTA1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 17521-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17521-1-AP (ACTA1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skeletal muscle using 17521-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17521-1-AP (ACTA1-Specific Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 17521-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17521-1-AP (ACTA1-Specific Antibody) at dilution of 1:500 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 17521-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17521-1-AP (ACTA1-Specific Antibody) at dilution of 1:500 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 17521-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 17521-1-AP (ACTA1-Specific Antibody) at dilution of 1:50.

• 

  IHC staining of human skeletal muscle using 17521-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 17521-1-AP (ACTA1-Specific Antibody) at dilution of 1:50.

• 

  IHC staining of human hysteromyoma using 17521-1-AP

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma using 17521-1-AP (ACTA1-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human hysteromyoma using 17521-1-AP

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma using 17521-1-AP (ACTA1-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  FC experiment of C2C12 using 17521-1-AP

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human ACTA1-Specific (17521-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive WB detected in: human skeletal muscle tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue
• Positive IHC detected in: human skeletal muscle tissue, human hysteromyoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: C2C12 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 7 publications below
• IF: See 3 publications below

Product Information

17521-1-AP targets ACTA1-Specific in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: actin, alpha 1, skeletal muscle
• Calculated molecular weight: 42 kDa
• Observed molecular weight: 39-42 kDa
• GenBank accession number: NM_001100
• Gene symbol: ACTA1
• Gene ID (NCBI): 58
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTA1 gene encodes skeletal muscle alpha-actin, the principal actin isoform in adult skeletal muscle, which interacts with a variety of proteins to produce the force for muscle contraction. There is only the N-termninal 8 amino acids difference among the actin family members. This antibody is specific to the ACTA1. It does not cross-react with other actin isoforms.

Publications

Species	Application	Title
mouse	IF	J Clin Invest; Impaired activity of the fusogenic micropeptide Myomixer causes myopathy resembling Carey-Fineman-Ziter syndrome.; Authors - Andres Ramirez-Martinez
human	WB	Cancer Lett; Cancer-associated fibroblasts-derived exosomal miR-17-5p promotes colorectal cancer aggressive phenotype by initiating a RUNX3/MYC/TGF-β1 positive feedback loop.; Authors - Yue Zhang
mouse	WB	Mol Ther Nucleic Acids; Diverging targets mediate the pathological roleof miR-199a-5p and miR-199a-3p by promoting cardiac hypertrophy and fibrosis; Authors - Ni Zeng
human	WB,IF	Cell Biosci; ACTA1 is inhibited by PAX3-FOXO1 through RhoA-MKL1-SRF signaling pathway and impairs cell proliferation, migration and tumor growth in Alveolar Rhabdomyosarcoma.; Authors - Qiande Hu
mouse	WB	J Cell Mol Med; Huoxin pill prevents acute myocardial ischaemia injury via inhibition of Wnt/β-catenin signaling.; Authors - Qing Wang
mouse	WB	Front Pharmacol; The Combination of Schisandrol B and Wedelolactone Synergistically Reverses Hepatic Fibrosis Via Modulating Multiple Signaling Pathways in Mice.; Authors - Yongqiang Ai

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C