ACTA1-Specific Polyclonal antibody
ACTA1-Specific Polyclonal Antibody for FC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IHC, IF, FC, ELISA
Cat no : 17521-1-AP
Validation Data Gallery
|Positive WB detected in||human skeletal muscle tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue|
|Positive IHC detected in||human skeletal muscle tissue, human hysteromyoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||C2C12 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
17521-1-AP targets ACTA1-Specific in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||actin, alpha 1, skeletal muscle|
|Calculated molecular weight||42 kDa|
|Observed molecular weight||39-42 kDa|
|GenBank accession number||NM_001100|
|Gene ID (NCBI)||58|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTA1 gene encodes skeletal muscle alpha-actin, the principal actin isoform in adult skeletal muscle, which interacts with a variety of proteins to produce the force for muscle contraction. There is only the N-termninal 8 amino acids difference among the actin family members. This antibody is specific to the ACTA1. It does not cross-react with other actin isoforms.
|Product Specific Protocols|
|WB protocol for ACTA1-Specific antibody 17521-1-AP||Download protocol|
|IHC protocol for ACTA1-Specific antibody 17521-1-AP||Download protocol|
|Click here to view our Standard Protocols|
J Clin Invest
Impaired activity of the fusogenic micropeptide Myomixer causes myopathy resembling Carey-Fineman-Ziter syndrome.
Cancer-associated fibroblasts-derived exosomal miR-17-5p promotes colorectal cancer aggressive phenotype by initiating a RUNX3/MYC/TGF-β1 positive feedback loop.
Mol Ther Nucleic Acids
Diverging targets mediate the pathological roleof miR-199a-5p and miR-199a-3p by promoting cardiac hypertrophy and fibrosis
ACTA1 is inhibited by PAX3-FOXO1 through RhoA-MKL1-SRF signaling pathway and impairs cell proliferation, migration and tumor growth in Alveolar Rhabdomyosarcoma.
J Cell Mol Med
Huoxin pill prevents acute myocardial ischaemia injury via inhibition of Wnt/β-catenin signaling.
The Combination of Schisandrol B and Wedelolactone Synergistically Reverses Hepatic Fibrosis Via Modulating Multiple Signaling Pathways in Mice.