ACTN2 Monoclonal antibody

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  WB analysis using 68223-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68223-1-Ig (ACTN2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat colon using 68223-1-Ig

  rat colon tissue were subjected to SDS PAGE followed by western blot with 68223-1-Ig (ACTN2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

ACTN2 Monoclonal Antibody for WB, ELISA

Cat No. 68223-1-Ig

Clone No.1D5F9

Publications(1)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, pig, rabbit

Applications

WB, ELISA

Synonyms

actinin, alpha 2, ACTN2, Alpha actinin 2, CMD1AA, F actin cross linking protein

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Tested Applications

• Positive WB detected in: pig heart tissue, rat colon tissue, rabbit heart tissue, rat heart tissue, mouse heart tissue, pig colon tissue

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

68223-1-Ig targets ACTN2 in WB, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: Human, mouse, rat, pig, rabbit
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: ACTN2 fusion protein Ag5578
• Full Name: actinin, alpha 2
• Calculated Molecular Weight: 104 kDa
• Observed Molecular Weight: 103 kDa
• GenBank Accession Number: BC051770
• Gene Symbol: ACTN2
• Gene ID (NCBI): 88
• RRID: AB_2935311
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P35609
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Alpha actinin 2 (ACTN2) belongs to the alpha-actinin family and is expressed in both skeletal and cardiac muscles and functions to anchor myofibrillar actin thin filaments and titin to Z-discs (PMID: 30701273). ACTN2 is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. Mutations in ACTN2 are associated with hypertrophic cardiomyopathy, as well as dilated cardiomyopathy and endocardial fibroelastosis (PMID: 20022194, 14567970).

Publications

Species	Application	Title
mouse	WB	iScience; Exercise training affects calcium ion transport by downregulating the CACNA2D1 protein to reduce hypertension-induced myocardial injury in mice; Authors - Shan Gao