ACTN2 Monoclonal antibody
ACTN2 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human, mouse, rat, pig, rabbit
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
1D5F9
Cat no : 68223-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | pig heart tissue, rat colon tissue, rabbit heart tissue, rat heart tissue, mouse heart tissue, pig colon tissue |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:20000-1:100000 |
Sample-dependent, check data in validation data gallery |
Product Information
68223-1-Ig targets ACTN2 in WB, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.
Tested Reactivity | Human, mouse, rat, pig, rabbit |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | ACTN2 fusion protein Ag5578 |
Full Name | actinin, alpha 2 |
Calculated molecular weight | 104 kDa |
Observed molecular weight | 103 kDa |
GenBank accession number | BC051770 |
Gene symbol | ACTN2 |
Gene ID (NCBI) | 88 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Alpha actinin 2 (ACTN2) belongs to the alpha-actinin family and is expressed in both skeletal and cardiac muscles and functions to anchor myofibrillar actin thin filaments and titin to Z-discs (PMID: 30701273). ACTN2 is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. Mutations in ACTN2 are associated with hypertrophic cardiomyopathy, as well as dilated cardiomyopathy and endocardial fibroelastosis (PMID: 20022194, 14567970).
Protocols
Product Specific Protocols | |
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WB protocol for ACTN2 antibody 68223-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |