ACTN2 Recombinant antibody, PBS Only (Capture)

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  WB analysis using 85857-4-RR (same clone as 85857-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85857-4-RR (ACTN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85857-4-PBS in a different storage buffer formulation.

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  IF Staining of H9C2 using 85857-4-RR (same clone as 85857-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using ACTN2 antibody (85857-4-RR, Clone: 250229F6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85857-4-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP02153-1

  Cytometric bead array standard curve of MP02153-1, ACTN2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85857-4-PBS. Detection antibody: 85857-3-PBS. Standard: Ag5459. Range: 0.781-100 ng/mL.

ACTN2 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 85857-4-PBS

Clone No.250229F6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

Actinin alpha 2, actinin, alpha 2, ACTN 2, Alpha actinin 2, Alpha-actinin skeletal muscle isoform 2

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Product Information

85857-4-PBS targets ACTN2 as part of a matched antibody pair:

MP02153-1: 85857-4-PBS capture and 85857-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ACTN2 fusion protein Ag5459
• Full Name: actinin, alpha 2
• Calculated Molecular Weight: 104 kDa
• Observed Molecular Weight: 103 kDa
• GenBank Accession Number: BC051770
• Gene Symbol: ACTN2
• Gene ID (NCBI): 88
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P35609
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Alpha actinin 2 (ACTN2) belongs to the alpha-actinin family and is expressed in both skeletal and cardiac muscles, and functions to anchor myofibrillar actin thin filaments and titin to Z-discs (PMID: 30701273). ACTN2 is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. Mutations in ACTN2 are associated with hypertrophic cardiomyopathy, as well as dilated cardiomyopathy and endocardial fibroelastosis (PMID: 20022194, 14567970).