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  • KD/KO Validated

ACTR10 Antibody

ACTR10 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20101-1-AP

Synonyms

Actin related protein 10, Actin related protein 11, ACTR10, ACTR11, ARP11, HARP11



Tested Applications

Positive WB detected inmouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20101-1-AP is ACTR10 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, Monkey, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACTR10 fusion protein Ag13612
Full Name actin-related protein 10 homolog (S. cerevisiae)
Calculated molecular weight 417 aa, 46 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC011997
Gene symbol ACTR10
Gene ID (NCBI) 55860
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ACTR10 antibody 20101-1-APDownload protocol
IHC protocol for ACTR10 antibody 20101-1-APDownload protocol
IF protocol for ACTR10 antibody 20101-1-APDownload protocol
IP protocol for ACTR10 antibody 20101-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
MonkeyWB

Mol Biol Cell

Dynactin's pointed-end complex is a cargo-targeting module.

Authors - Ting-Yu Yeh
  • KD Validated
humanWB

Curr Biol

An RNAi Screen in a Novel Model of Oriented Divisions Identifies the Actin-Capping Protein Z β as an Essential Regulator of Spindle Orientation.

Authors - Florencia di Pietro
ratWB

Proc Natl Acad Sci U S A

Dynein/dynactin is necessary for anterograde transport of Mbp mRNA in oligodendrocytes and for myelination in vivo.

Authors - Amy L Herbert