ARP2 Polyclonal antibody

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• ARP2 Polyclonal antibody (10922-1-AP)

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• KD/KO Validated

ARP2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 10922-1-AP Copied!

Publications (24)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

ACTR2, Actin-like protein 2, Actin-related protein 2

• 

  WB analysis using 10922-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10922-1-AP (ARP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse kidney using 10922-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10922-1-AP (ARP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human prostate cancer using 10922-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10922-1-AP (ARP2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human prostate cancer using 10922-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10922-1-AP (ARP2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 10922-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10922-1-AP (ARP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human prostate cancer using 10922-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10922-1-AP (ARP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human prostate cancer using 10922-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10922-1-AP (ARP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of U-251 using 10922-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using ARP2 antibody (10922-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of HEK-293 using 10922-1-AP

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human ARP2 (10922-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, mouse kidney tissue, Jurkat cells, MCF-7 cells, SH-SY5Y cells
• Positive IHC detected in: human prostate cancer tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U-251 cells
• Positive FC (Intra) detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10922-1-AP targets ARP2 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1359

  Product name: Recombinant human ACTR2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-394 aa of BC014546

  Sequence: MDSQGRKVVVCDNGTGFVKCGYAGSNFPEHIFPALVGRPIIRSTTKVGNIEIKDLMVGDEASELRSTLEVNYPMENGIVRNWDDMKHLWDYTFGPEKLNIDTRNCKILLTEPPMNPTKNREKIVEVMFETYQFSGVYVAIQAVLTLYAQGLLTGVVVDSGDGVTHICPVYESFSLPHLTRRLDIAGRDITRYLIKLLLLRGYAFNHSADFETVRMIKEKLCYVGYNIEQEQKLALETTVLVESYTLPDGRIIKVGGERFEAPEALFQPHLINVEGVGVAELLFNTIQAADIDTRSEFYKHIVLSGGSTMYPGLPSRLERELKQLYLERVLKGDVEKLSKFKIRIEDPPRRKHMVFLGGAVLADIMKDKDNFWMTRQEYQEKGVRVLEKLGVTVR

• Full Name: ARP2 actin-related protein 2 homolog (yeast)
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 43-45 kDa
• GenBank Accession Number: BC014546
• Gene Symbol: ARP2/ACTR2
• Gene ID (NCBI): 10097
• RRID: AB_2221854
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P61160
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The anti-β-Actin antibody (Cat# 10922-1-AP) has accumulated 24 citations across journals including Nature Cell Biology, Nature Communications, Nucleic Acids Research, Advanced Science, Cancer Letters, Cell Reports, EMBO Reports, eLife, Journal of Biological Chemistry, and others. Associated research fields encompass actin cytoskeleton dynamics, cancer metastasis, reproductive biology (oocyte and embryo development), neuroscience, renal fibrosis, vascular biology, virology, and cellular trafficking.

Automatically generated from the 24 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Cell Biol; miR-129-3p controls cilia assembly by regulating CP110 and actin dynamics.; Authors - Cao Jingli J ; KD Validated
		Nat Commun; Synergistic IL-6 and IL-8 paracrine signalling pathway infers a strategy to inhibit tumour cell migration.; Authors - Hasini Jayatilaka
human	WB	Nucleic Acids Res; G3BP1 binds to guanine quadruplexes in mRNAs to modulate their stabilities.; Authors - Xiaomei He
mouse	WB,IF	Adv Sci (Weinh); Microfilament-Myosin II Regulates the Differentiation of Multinucleated Cysts into Oocytes and Influences Oocyte Developmental Potential in Mice.; Authors - Rui Xu
human	WB,IHC	Cancer Lett; Sulforaphane suppresses bladder cancer metastasis via blocking actin nucleation-mediated pseudopodia formation; Authors - Lei Huang
		Neural Regen Res; Disulfidptosis contributes to rotenone-induced dopaminergic neuron damage.; Authors - Wenqi Ye