|Positive WB detected in||HeLa cells, mouse kidney tissue|
|Positive IHC detected in||human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HEK-293 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:200-1:800|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10922-1-AP targets ARP2 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ARP2 fusion protein Ag1359|
|Full Name||ARP2 actin-related protein 2 homolog (yeast)|
|Calculated molecular weight||45 kDa|
|Observed molecular weight||43-45 kDa|
|GenBank accession number||BC014546|
|Gene ID (NCBI)||10097|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Inhibition of N-WASP affects actin-mediated cytokinesis during porcine oocyte maturation.
RAB8A GTPase regulates spindle migration and Golgi apparatus distribution via ROCK-mediated actin assembly in mouse oocyte meiosis.
mTOR signaling regulates the morphology and migration of outer radial glia in developing human cortex.
Nat Cell Biol
miR-129-3p controls cilia assembly by regulating CP110 and actin dynamics.