ACVRL1 Monoclonal antibody, PBS Only

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  WB analysis of HUVEC using 66857-1-Ig (same clone as 66857-1-PBS)

  HUVEC cells were subjected to SDS PAGE followed by western blot with 66857-1-Ig (ACVRL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66857-1-PBS in a different storage buffer formulation.

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  WB analysis of JAR using 66857-1-Ig (same clone as 66857-1-PBS)

  JAR cells were subjected to SDS PAGE followed by western blot with 66857-1-Ig (ACVRL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66857-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 66857-1-Ig (same clone as 66857-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66857-1-Ig (ACVRL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66857-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 66857-1-Ig (same clone as 66857-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66857-1-Ig (ACVRL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66857-1-PBS in a different storage buffer formulation.

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  WB analysis of Daudi using 66857-1-Ig (same clone as 66857-1-PBS)

  Daudi cells were subjected to SDS PAGE followed by western blot with 66857-1-Ig (ACVRL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66857-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66857-1-Ig (same clone as 66857-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66857-1-Ig (ACVRL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66857-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 66857-1-Ig (same clone as 66857-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66857-1-Ig (ACVRL1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66857-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 66857-1-Ig (same clone as 66857-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66857-1-Ig (ACVRL1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66857-1-PBS in a different storage buffer formulation.

ACVRL1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 66857-1-PBS

Clone No.2E11B10

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

2E11B10, Activin receptor like kinase 1, Activin receptor-like kinase 1, ACVRLK1, ALK 1

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Product Information

66857-1-PBS targets ACVRL1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6446

  Product name: Recombinant human ACVRL1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 142-503 aa of BC042637

  Sequence: HVRRRQEKQRGLHSELGESSLILKASEQGDSMLGDLLDSDCTTGSGSGLPFLVQRTVARQVALVECVGKGRYGEVWRGLWHGESVAVKIFSSRDEQSWFRETEIYNTVLLRHDNILGFIASDMTSRNSSTQLWLITHYHEHGSLYDFLQRQTLEPHLALRLAVSAACGLAHLHVEIFGTQGKPAIAHRDFKSRNVLVKSNLQCCIADLGLAVMHSQGSDYLDIGNNPRVGTKRYMAPEVLDEQIRTDCFESYKWTDIWAFGLVLWEIARRTIVNGIVEDYRPPFYDVVPNDPSFEDMKKVVCVDQQTPTIPNRLAADPVLSGLAQMMRECWYPNPSARLTALRIKKTLQKISNSPEKPKVIQ

• Full Name: activin A receptor type II-like 1
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 56 kDa, 63 kDa
• GenBank Accession Number: BC042637
• Gene Symbol: ACVRL1
• Gene ID (NCBI): 94
• RRID: AB_2882196
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P37023
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ACVRL1 (also known as ALK1) is a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. ACVRL1 is highly expressed in endothelial cells and has a critical role in the control of blood vessel development and repair (PMID: 8640225). Mutations in the ACVRL1 gene are associated with hemorrhagic telangiectasia type 2.