ADAM15 Polyclonal antibody

ADAM15 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27124-1-AP

Synonyms

ADAM 15, ADAM15, MDC 15, MDC15, Metargidin



Tested Applications

Positive WB detected inA549 cells, HUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 27124-1-AP is ADAM15 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADAM15 fusion protein Ag25947
Full Name ADAM metallopeptidase domain 15
Calculated Molecular Weight 93 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC014566
Gene Symbol ADAM15
Gene ID (NCBI) 8751
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADAM15, a member of the ADAM (a disintegrin and metalloprotease) family, is a membrane protein containing both protease and adhesion domains and may be involved in tumor invasion and metastasis (PMID:15756594). It is also named as MDC15. ADAM15 is required for the activation of the FAK and Src pathways to generate apoptosis resistance in response to apoptotic signalling or cell stress (PMID: 11741929). ADAM15 is processed during epididymal maturation and acrosome reaction and that it may play a role during sperm-egg binding. Jurkat and testicular cells expresses the precursor ADAM15 of 110 kDa, as well as the ADAM15 processing form, consisting of about 75-85 kDa (PMID:18390692). ADAM15 has some isoforms with the calculated molecular mass of 55~92 kDa.

Protocols

Product Specific Protocols
WB protocol for ADAM15 antibody 27124-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols