CoraLite® Plus 488-conjugated ADAMTS2 Recombinant antibody

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  IF Staining of A431 using CL488-84476-6

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 ADAMTS2 antibody (CL488-84476-6, Clone: 241860B8 ) at dilution of 1:200.

ADAMTS2 Recombinant Antibody for IF/ICC

Cat No. CL488-84476-6

Clone No.241860B8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

ADAMTS-2, 241860B8, ADAMTS 2, PCINP, PCPNI

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Tested Applications

• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-84476-6 targets ADAMTS2 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ADAMTS2 fusion protein Ag34957
• Full Name: ADAM metallopeptidase with thrombospondin type 1 motif, 2
• Calculated Molecular Weight: 135 kDa
• Observed Molecular Weight: 110~130 kDa
• GenBank Accession Number: NM_014244
• Gene Symbol: ADAMTS2
• Gene ID (NCBI): 9509
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O95450
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

A disintegrin and metalloproteinase with thrombospondin motifs 2 (ADAMTS-2) is a metalloproteinase that plays a key role in the processing of fibrillar procollagen precursors into mature collagen molecules by excising the amino-propeptide. ADAMTS-2 can, like the procollagen C-proteinases, be regulated by transforming growth factor-β1 (TGF-β1), with implications for mechanisms whereby this growth factor effects net increases in formation of extracellular matrix. Mutations in the ADAMTS-2 gene cause dermatosparaxis in human (dermatosparactic type of Ehlers-Danlos syndrome, also known as Ehlers-Danlos syndrome type VIIC). Human ADAMTS-2 has eight potential sites for Asn-linked glycosylation and other mammals (PMID: 16046392, 12646579).