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ADCY5 Polyclonal antibody, PBS Only (30153-1-PBS)

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WB analysis using 30153-1-AP (same clone as 30153-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 30153-1-AP (ADCY5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30153-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 30153-1-AP (same clone as 30153-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 30153-1-AP (ADCY5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30153-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 30153-1-AP (same clone as 30153-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 30153-1-AP (ADCY5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30153-1-PBS in a different storage buffer formulation.
IF Staining of mouse brain using 30153-1-AP (same clone as 30153-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ADCY5 antibody (30153-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30153-1-PBS in a different storage buffer formulation.
IF Staining of mouse brain using 30153-1-AP (same clone as 30153-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ADCY5 antibody (30153-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30153-1-PBS in a different storage buffer formulation.

ADCY5 Polyclonal antibody, PBS Only

Name: ADCY5 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 30153-1-PBS

ADCY5 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 30153-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

AC5, adenylate cyclase 5, Adenylate cyclase type 5, Adenylate cyclase type V

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

30153-1-PBS targets ADCY5 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag32812 Product name: Recombinant human ADCY5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-195 aa of NM_183357 Sequence: MSGSKSVSPPGYAAQKTAAPAPRGGPEHRSAWGEADSRANGYPHAPGGSARGSTKKPGGAVTPQQQQRLASRWRSDDDDDPPLSGDDPLAGGFGFSFRSKSAWQERGGDDCGRGSRRQRRGAASGGSTRAPPAGGGGGSAAAAASAGGTEVRPRSVEVGLEERRGKGRAADELEAGAVEGGEGSGDGGSSADSGS Predict reactive species
Full Name	adenylate cyclase 5
Calculated Molecular Weight	139 kDa
Observed Molecular Weight	~150 kDa
GenBank Accession Number	NM_183357
Gene Symbol	ADCY5
Gene ID (NCBI)	111
RRID	AB_3086248
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O95622
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

ADCY5 belongs to the adenylate cyclase (ADCY) superfamily which is a group of glycoproteins regulating intracellular signaling (PMID: 35832555). ADCY5 is expressed only in neurons and all neurons express ADCY5 (PMID: 31970214). Gain-of-function mutations in ADCY5 is linked to a rare movement-onset disorder called ADCY5-related dyskinesia (PMID: 32627162).