Alpha Adducin Polyclonal antibody

Alpha Adducin Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 10791-1-AP

Synonyms

ADD1, ADDA, adducin 1 (alpha), Alpha adducin

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Validation Data Gallery

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  WB analysis of K-562 using 10791-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10791-1-AP (Alpha adducin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 10791-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10791-1-AP (Alpha adducin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 10791-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10791-1-AP (Alpha adducin antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary tumor using 10791-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10791-1-AP (Alpha adducin antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of mouse brain using 10791-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Alpha Adducin antibody (10791-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: K-562 cells, mouse testis tissue
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

10791-1-AP targets Alpha Adducin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: rat, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Alpha Adducin fusion protein Ag1245
• Full Name: adducin 1 (alpha)
• Calculated Molecular Weight: 81 kDa, 84 kDa, 70 kDa
• Observed Molecular Weight: 115-120 kDa
• GenBank Accession Number: BC013393
• Gene Symbol: ADD1
• Gene ID (NCBI): 118
• RRID: AB_2257952
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	WB	Int J Mol Sci; Decoding the Synaptic Proteome with Long-Term Exposure to Midazolam during Early Development.; Authors - Nghi M Nguyen
bovine	WB	Mol Biol Rep; Depletion of serum-derived exosomes aggravates heat stress-induced damage of bovine mammary epithelial cells; Authors - Yue Wang