Gamma Adducin Polyclonal antibody

• Featured Product
• KD/KO Validated

Gamma Adducin Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 17585-1-AP

Synonyms

ADD3, ADDL, adducin 3 (gamma), Adducin like protein 70, Gamma adducin

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Validation Data Gallery

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  WB analysis of NIH/3T3 using 17585-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 17585-1-AP (Gamma adducin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 17585-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17585-1-AP (Gamma adducin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 17585-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17585-1-AP (Gamma adducin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of NIH/3T3 using 17585-1-AP

  IP result of anti-Gamma Adducin (IP:17585-1-AP, 4ug; Detection:17585-1-AP 1:500) with NIH/3T3 cells lysate 4000ug.

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, K-562 cells, mouse kidney tissue
• Positive IP detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below

Product Information

17585-1-AP targets Gamma Adducin in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Gamma Adducin fusion protein Ag11318
• Full Name: adducin 3 (gamma)
• Calculated Molecular Weight: 76 kDa, 79 kDa
• Observed Molecular Weight: 80 kDa, 90 kDa
• GenBank Accession Number: BC062559
• Gene Symbol: Gamma Adducin
• Gene ID (NCBI): 120
• RRID: AB_2223579
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	J Mol Cell Biol; QKI-5 regulates the alternative splicing of cytoskeletal gene ADD3 in lung cancer.; Authors - Jin-Zhu Wang
human	WB	Stem Cell Res Ther; Knockdown of long noncoding RNA SAN rejuvenates aged adipose-derived stem cells via miR-143-3p/ADD3 axis; Authors - Hewei Xiong ; KD Validated
human	WB	PLoS One; Dysferlin and other non-red cell proteins accumulate in the red cell membrane of diamond-blackfan anemia patients.; Authors - Esther N Pesciotta
human	WB	Cell Genom; Protein interaction studies in human induced neurons indicate convergent biology underlying autism spectrum disorders; Authors - Greta Pintacuda