ADH1C Polyclonal antibody

ADH1C Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF,ELISA

Conjugate

Unconjugated

Cat no : 18897-1-AP

Synonyms

ADH1C, Alcohol dehydrogenase 1C



Tested Applications

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

18897-1-AP targets ADH1C in WB, IP, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADH1C fusion protein Ag13528
Full Name alcohol dehydrogenase 1C (class I), gamma polypeptide
Calculated molecular weight 375 aa, 40 kDa
Observed molecular weight 37-45 kDa
GenBank accession numberBC062476
Gene symbol ADH1C
Gene ID (NCBI) 126
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ADH1C(Alcohol dehydrogenase 1C) is also named as ADH3 and belongs to the zinc-containing alcohol dehydrogenase family.It catalyzes the rate-limiting step for ethanol metabolism: the oxidation of alcohol to acetaldehyde.The ADH1C gene is active in intestine and kidney in fetal and early postnatal life, and persists in the stomach and liver in adult life(PMID:4748759).

Protocols

Product Specific Protocols
WB protocol for ADH1C antibody 18897-1-APDownload protocol
IF protocol for ADH1C antibody 18897-1-APDownload protocol
IP protocol for ADH1C antibody 18897-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Free Radic Biol Med

PPARα agonist WY-14,643 enhances ethanol metabolism in mice: role of catalase.

Authors - Xue Chen