ADHFE1 Polyclonal antibody

ADHFE1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 14674-1-AP

Synonyms

HOT, HMFT2263, Fe-containing alcohol dehydrogenase, EC:1.1.99.24, Alcohol dehydrogenase iron-containing protein 1

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Validation Data Gallery

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  WB analysis using 14674-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14674-1-AP (ADHFE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 14674-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14674-1-AP (ADHFE1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 14674-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14674-1-AP (ADHFE1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver using 14674-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14674-1-AP (ADHFE1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 14674-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14674-1-AP (ADHFE1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon cancer using 14674-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14674-1-AP (ADHFE1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 14674-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14674-1-AP (ADHFE1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HT-29 cells, mouse kidney tissue, mouse liver tissue, rat kidney tissue
• Positive IHC detected in: human colon tissue, human colon cancer tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14674-1-AP targets ADHFE1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ADHFE1 fusion protein Ag6382
• Full Name: alcohol dehydrogenase, iron containing, 1
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 50 kDa, 40-45 kDa
• GenBank Accession Number: BC064634
• Gene Symbol: ADHFE1
• Gene ID (NCBI): 137872
• RRID: AB_2221451
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The ADHFE1 gene encodes hydroxyacid-oxoacid transhydrogenase which is responsible for the oxidation of 4-hydroxybutyrate in mammalian tissues. It belongs to the iron-containing alcohol dehydrogenase family. ADHFE1 has 4 isoforms produced by alternative splicing with the MW of 50, 45, 32, 27 kDa.

Publications

Species	Application	Title
		Circ Cardiovasc Genet; Proteome-Based Systems Biology Analysis of the Diabetic Mouse Aorta Reveals Major Changes in Fatty Acid Biosynthesis as Potential Hallmark in Diabetes Associated Vascular Disease.; Authors - Holger Husi