ADK Monoclonal antibody

ADK Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1G1A5

Cat no : 66929-1-Ig

Synonyms

Adenosine 5 phosphotransferase, adenosine kinase, ADK, AK



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman liver cancer tissue, human breast cancer tissue
Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:30000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

66929-1-Ig targets ADK in WB, IHC, IF,ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ADK fusion protein Ag7466
Full Name adenosine kinase
Calculated molecular weight 41 kDa
Observed molecular weight 40-46 kDa
GenBank accession numberBC003568
Gene symbol ADK
Gene ID (NCBI) 132
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ADK antibody 66929-1-IgDownload protocol
IHC protocol for ADK antibody 66929-1-IgDownload protocol
IF protocol for ADK antibody 66929-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols