ADK Monoclonal antibody, PBS Only

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  WB analysis using 66929-1-Ig (same clone as 66929-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66929-1-Ig (ADK antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66929-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66929-1-Ig (same clone as 66929-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66929-1-Ig (ADK antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66929-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66929-1-Ig (same clone as 66929-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66929-1-Ig (ADK antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66929-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 66929-1-Ig (same clone as 66929-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using ADK antibody (66929-1-Ig, Clone: 1G1A5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66929-1-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 66929-1-Ig (same clone as 66929-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using ADK antibody (66929-1-Ig, Clone: 1G1A5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66929-1-PBS in a different storage buffer formulation.

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  FC experiment of NIH/3T3 using 66929-1-Ig (same clone as 66929-1-PBS)

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug Anti-Human ADK (66929-1-Ig, Clone:1G1A5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66929-1-PBS in a different storage buffer formulation.

ADK Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA

Cat No. 66929-1-PBS

Clone No.1G1A5

Host / Isotype

Mouse / IgG1

Reactivity

rat, mouse, human

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Synonyms

EC:2.7.1.20, AK, adenosine kinase, Adenosine 5'-phosphotransferase, 1G1A5

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Product Information

66929-1-PBS targets ADK in WB, IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with rat, mouse, human samples.

• Tested Reactivity: rat, mouse, human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7466

  Product name: Recombinant human ADK protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-345 aa of BC003568

  Sequence: MTSVRENILFGMGNPLLDISAVVDKDFLDKYSLKPNDQILAEDKHKELFDELVKKFKVEYHAGGSTQNSIKVAQWMIQQPHKAATFFGCIGIDKFGEILKRKAAEAHVDAHYYEQNEQPTGTCAACITGDNRSLIANLAAANCYKKEKHLDLEKNWMLVEKARVCYIAGFFLTVSPESVLKVAHHASENNRIFTLNLSAPFFSQFYKESLMKVMPYVDILFGNETEAATFAREQGFETKDIKEIAKKTQALPKMNSKRQRIVIFTQGRDDTIMATESEVTAFAVLDQDQKEIIDTNGAGDAFVGGFLSQLVSDKPLTECIRAGHYAASIIIRRTGCTFPEKPDFH

• Full Name: adenosine kinase
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 40-46 kDa
• GenBank Accession Number: BC003568
• Gene Symbol: ADK
• Gene ID (NCBI): 132
• RRID: AB_2882255
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P55263
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.