AE2 Polyclonal antibody

AE2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 26332-1-AP

Synonyms

AE 2, AE2, Anion exchange protein 2, Anion exchanger 2, BND3L, EPB3L1, HKB3, MPB3L, NBND3, SLC4A2

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Validation Data Gallery

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  WB analysis using 26332-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26332-1-AP (AE2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 26332-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26332-1-AP (AE2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 26332-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26332-1-AP (AE2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 26332-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26332-1-AP (AE2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 26332-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26332-1-AP (AE2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells
• Positive IHC detected in: human liver cancer tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

26332-1-AP targets AE2 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AE2 fusion protein Ag23673
• Full Name: solute carrier family 4, anion exchanger, member 2 (erythrocyte membrane protein band 3-like 1)
• Calculated Molecular Weight: 1241 aa, 137 kDa
• Observed Molecular Weight: 150 and 70 kDa
• GenBank Accession Number: BC028601
• Gene Symbol: AE2
• Gene ID (NCBI): 6522
• RRID: AB_2880481
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC4A2, also named as AE2, encodes a Cl-/HCO3- exchanger. It has been reported that the reduced expression of SLC4A2 found in the liver and peripheral blood mononuclear cells of patients may be associated with the hypermethylation of SLC4A2 promoter regions, which might contribute to the pathogenesis of primary biliary cholangitis (PBC). 26332-1-AP antibody detects 150 kDa and 70 kDa (XP_006716157, 666aa) protein in SDS-PAGE. (PMID: 23341620, 32039364)

Publications

Species	Application	Title
human	WB	Mol Cell; The mTORC1-SLC4A7 axis stimulates bicarbonate import to enhance de novo nucleotide synthesis.; Authors - Eunus S Ali