AEG-1/MTDH Monoclonal antibody

AEG-1/MTDH Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1D8C2

Cat no : 68592-1-Ig

Synonyms

3D3, 3D3/LYRIC, AEG 1, AEG1, LYRIC, LYRIC/3D3, metadherin, Metastasis adhesion protein, MTDH, Protein LYRIC

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Validation Data Gallery

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  WB analysis using 68592-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68592-1-Ig (AEG-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A431 cells, MCF-10A cells, A549 cells, LNCaP cells, HeLa cells, Jurkat cells, MOLT-4 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68592-1-Ig targets AEG-1/MTDH in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: AEG-1/MTDH fusion protein Ag5033
• Full Name: metadherin
• Calculated Molecular Weight: 582 aa, 64 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC045642
• Gene Symbol: MTDH
• Gene ID (NCBI): 92140
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MTDH, also known as AEG-1, is a single-pass transmembrane protein with its gene located at chromosome 8q22 , the abnormalities of which have been identified in several tumor types. MTDH is overexpressed in a number of malignancies, such as breast cancer, malignant glioma, and neuroblastoma , and its overexpression is associated with poor clinical outcome. MTDH plays an important role in the regulation of carcinogenesis and tumor progression.