AES Monoclonal antibody

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  WB analysis using 67869-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67869-1-Ig (AES antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

AES Monoclonal Antibody for WB, ELISA

Cat No. 67869-1-Ig

Clone No.1F7E1

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Synonyms

AES, AES 1, AES 2, Amino enhancer of split, ESP1, Gp130 associated protein GAM, GRG, GRG5, Protein ESP1, Protein GRG, TLE5

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Tested Applications

• Positive WB detected in: HCT 116 cells, PC-3 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, MOLT-4 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67869-1-Ig targets AES in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag31320

  Product name: Recombinant human AES protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-81 aa of BC113735

  Sequence: MCHKNGFPQEGGITAAFLQKRKLRLSKNHRPARAKVTEHVRGTRPGRATAGPAASTRAAGSLFFDRWGNRGPAGCRGSSHL

• Full Name: amino-terminal enhancer of split
• Calculated Molecular Weight: 264 aa, 29 kDa
• Observed Molecular Weight: 29-31 kDa
• GenBank Accession Number: BC113735
• Gene Symbol: AES
• Gene ID (NCBI): 166
• RRID: AB_2918627
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q08117
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.