AF-6 Polyclonal antibody, PBS Only

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• AF-6 Polyclonal antibody, PBS Only (55102-1-PBS)

• 

  WB analysis of mouse kidney using 55102-1-AP (same clone as 55102-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 55102-1-AP (AF-6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55102-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 55102-1-AP (same clone as 55102-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 55102-1-AP (AF-6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55102-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 55102-1-AP (same clone as 55102-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 55102-1-AP (AF-6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55102-1-PBS in a different storage buffer formulation.

AF-6 Polyclonal Antibody for WB, Indirect ELISA

Cat No. 55102-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

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Product Information

55102-1-PBS targets AF-6 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 4
• Calculated Molecular Weight: 207 kDa
• Observed Molecular Weight: 182-210 kDa
• GenBank Accession Number: NM_005936
• Gene Symbol: Afadin/AF6
• Gene ID (NCBI): 4301
• RRID: AB_10792809
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P55196
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

MLLT4, also named as AF6 and Afadin, belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). It is a nNectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton. The antibody is specific to MLLT4.