• Featured Product
  • KD/KO Validated

AFAP1 Polyclonal antibody

AFAP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 21093-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

110 kDa actin filament-associated protein, Actin filament-associated protein 1, AFAP, AFAP 110, AFAP-110

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inDU 145 cells, mouse brain tissue, rat brain tissue, HeLa cells, MCF-7 cells, PC-3 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, A549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21093-1-AP targets AFAP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag15346

Product name: Recombinant human AFAP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 382-730 aa of BC032777

Sequence: DLKTHIVSIPLRGCEVIPGLDCKHPLTFRLLRNGQEVAVLEASSSEDMGRWIGILLAETGSSTDPEALHYDYIDVEMSASVIQTAKQTFCFMNRRVISANPYLGGTSNGYAHPSGTALHYDDVPCINGSLKGKKPPVASNGVTGKGKTLSSQPKKADPAAVVKRTGSNAAQYKYGKNRVEADAKRLQTKEEELLKRKEALRNRLAQLRKERKDLRAAIEVNAGRKPQAILEEKLKQLEEECRQKEAERVSLELELTEVKESLKKALAGGVTLGLAIEPKSGTSSPQSPVFRHRTLENSPISSCDTSDTEGPVPVNSAAVLKKSQAAPGSSPCRGHVLRKAKEWELKNGT

Predict reactive species
Full Name actin filament associated protein 1
Calculated Molecular Weight 730 aa, 81 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC032777
Gene Symbol AFAP1
Gene ID (NCBI) 60312
RRIDAB_10732810
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N556
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Actin filament-associated protein 1 (AFAP1) encodes a motor fiber-associated protein, which organizes a network for linking other proteins, including SRC proto-oncogene, non-receptor tyrosine kinase (Src) and protein kinase C . A long non-coding RNA, namely AFAP1-antisense RNA 1 (AS1), is transcribed from the antisense strand of this gene and potentially regulates its expression.(PMID: 31086663, PMID: 35193469)

Protocols

Product Specific Protocols
WB protocol for AFAP1 antibody 21093-1-APDownload protocol
IHC protocol for AFAP1 antibody 21093-1-APDownload protocol
IF protocol for AFAP1 antibody 21093-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nucleic Acids Res

CSNK2B modulates IRF1 binding to functional DNA elements and promotes basal and agonist-induced antiviral signaling

Authors - Moe Matsumoto
  • KD Validated
WB,IF

Mol Biol Cell

Spatial proximity of proteins surrounding zyxin under force-bearing conditions.

Authors - Joleen S Cheah
  • KD Validated
ratWB

Int J Mol Med

Curculigoside attenuates myocardial ischemia‑reperfusion injury by inhibiting the opening of the mitochondrial permeability transition pore.

Authors - Yanbing Zhao
humanWB

Biol Res

Long non-coding RNA AFAP1-AS1 facilitates tumor growth and promotes metastasis in colorectal cancer.

Authors - Xu Han
humanWB

J Exp Clin Cancer Res

LncRNA AFAP1-AS1 promotes tumorigenesis and epithelial-mesenchymal transition of osteosarcoma through RhoC/ROCK1/p38MAPK/Twist1 signaling pathway.

Authors - Deyao Shi
humanWB

Oncotarget

LncRNA AFAP1-AS1 promotes growth and metastasis of cholangiocarcinoma cells.

Authors - Xiuhui Shi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Joleen (Verified Customer) (05-04-2019)

Works excellently for WB. Strong signal by 5s exposure and with typical dilution.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1k
  • Cell Tissue Type: MDCK GII
AFAP1 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:21093-1-AP)
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