AFAP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

AFAP1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Reviews (1)

Cat no : 21093-1-AP

Synonyms

AFAP, AFAP 110, AFAP1

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Validation Data Gallery

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  WB analysis using 21093-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21093-1-AP (AFAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 21093-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21093-1-AP (AFAP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 21093-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 21093-1-AP (AFAP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 21093-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21093-1-AP (AFAP1 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 21093-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21093-1-AP (AFAP1 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 21093-1-AP

  Immunofluorescent analysis of MCF-7 cells using 21093-1-AP (AFAP1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: DU 145 cells, mouse brain tissue, rat brain tissue, HeLa cells, MCF-7 cells, PC-3 cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IF: See 1 publications below

Product Information

21093-1-AP targets AFAP1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AFAP1 fusion protein Ag15346
• Full Name: actin filament associated protein 1
• Calculated Molecular Weight: 730 aa, 81 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC032777
• Gene Symbol: AFAP1
• Gene ID (NCBI): 60312
• RRID: AB_10732810
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Actin filament-associated protein 1 is a protein that in humans is encoded by the AFAP1 gene. The protein encoded by this gene is a Src binding partner. It may represent a potential modulator of actin filament integrity in response to cellular signals, and may function as an adaptor protein by linking Src family members and/or other signaling proteins to actin filaments.

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; CSNK2B modulates IRF1 binding to functional DNA elements and promotes basal and agonist-induced antiviral signaling; Authors - Moe Matsumoto ; KD Validated
	WB, IF	Mol Biol Cell; Spatial proximity of proteins surrounding zyxin under force-bearing conditions.; Authors - Joleen S Cheah ; KD Validated
rat	WB	Int J Mol Med; Curculigoside attenuates myocardial ischemia‑reperfusion injury by inhibiting the opening of the mitochondrial permeability transition pore.; Authors - Yanbing Zhao
human	WB	Biol Res; Long non-coding RNA AFAP1-AS1 facilitates tumor growth and promotes metastasis in colorectal cancer.; Authors - Xu Han
human	WB	J Exp Clin Cancer Res; LncRNA AFAP1-AS1 promotes tumorigenesis and epithelial-mesenchymal transition of osteosarcoma through RhoC/ROCK1/p38MAPK/Twist1 signaling pathway.; Authors - Deyao Shi
human	WB	Oncotarget; LncRNA AFAP1-AS1 promotes growth and metastasis of cholangiocarcinoma cells.; Authors - Xiuhui Shi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Joleen (Verified Customer) (05-04-2019)

Works excellently for WB. Strong signal by 5s exposure and with typical dilution.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

AFAP1 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:21093-1-AP)