Tested Applications
| Positive WB detected in | A549 cells, mouse thymus tissue |
| Positive IP detected in | mouse thymus tissue |
| Positive IHC detected in | human thymus tissue, human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17183-1-AP targets AFAP1L2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10966 Product name: Recombinant human AFAP1L2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 466-818 aa of BC033212 Sequence: SCIVSAAKNSLLLMQRKFSEPNTYIDGLPSQDRQEELYDDVDLSELTAAVEPTEEATPVADDPNERESDRVYLDLTPVKSFLHGPSSAQAQASSPTLSCLDNATEALPADSGPGPTPDEPCIKCPENLGEQQLESLEPEDPSLRITTVKIQTEQQRISFPPSCPDAVVATPPGASPPVKDRLRVTSAEIKLGKNRTEAEVKRYTEEKERLEKKKEEIRGHLAQLRKEKRELKETLLKCTDKEVLASLEQKLKEIDEECRGEESRRVDLELSIMEVKDNLKKAEAGPVTLGTTVDTTHLENVSPRPKAVTPASAPDCTPVNSATTLKNRPLSVVVTGKGTVLQKAKEWEKKGAS Predict reactive species |
| Full Name | actin filament associated protein 1-like 2 |
| Calculated Molecular Weight | 818 aa, 91 kDa |
| Observed Molecular Weight | 130 kDa |
| GenBank Accession Number | BC033212 |
| Gene Symbol | AFAP1L2 |
| Gene ID (NCBI) | 84632 |
| RRID | AB_2226101 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N4X5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
AFAP1L2, also named as Actin filament-associated protein 1-like 2 or XB130, is a 818 amino acid protein, which contains two PH domains. AFAP1L2 is detected in spleen and thyroid, and at lower levels in kidney, brain, lung and pancreas. AFAP1L2 localizes in the cytoplasm and may play a role in a signaling cascade by enhancing the kinase activity of SRC. AFAP1L2 promotes thyroid tumor growth, and its expression in these cancer cells may affect cell proliferation and survival by controlling the expression of multiple genes, especially transcription regulators.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for AFAP1L2 antibody 17183-1-AP | Download protocol |
| IP protocol for AFAP1L2 antibody 17183-1-AP | Download protocol |
| WB protocol for AFAP1L2 antibody 17183-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The AFAP1L2 (XB130) polyclonal antibody (Cat# 17183-1-AP) has 8 citations published in journals including Autophagy, Molecular Cell, Cell Reports, Cancer Cell International, Translational Oncology, Cell Signaling, Carcinogenesis, and Oncology Letters. Research fields span hepatocellular carcinoma and mitophagy, prostate cancer PI3K signaling, Alzheimer's disease-related brain endothelial biology, non-small cell lung cancer progression, soft tissue tumor metastasis, and skin tumorigenesis.
| Species | Application | Title |
|---|---|---|
Autophagy Artesunate Sensitizes human hepatocellular carcinoma to sorafenib via exacerbating AFAP1L2-SRC-FUNDC1 axis-dependent mitophagy | ||
Cell Rep Integrative multi-omic profiling of adult mouse brain endothelial cells and potential implications in Alzheimer's disease | ||
Cancer Cell Int Heterogeneous nuclear ribonucleoprotein C promotes non-small cell lung cancer progression by enhancing XB130 mRNA stability and translation | ||
Transl Oncol Identification of genes associated with local aggressiveness and metastatic behavior in soft tissue tumors. | ||
Cell Signal HnRNPR promotes non-small cell lung cancer progression by protecting XB130 mRNA from XRN1- and DIS3L2-mediated degradation |
Reviews
What customers say
One reviewer (Tamara Chessa, Dec 2020) rated this antibody 5/5 stars, reporting that it works very well for detecting endogenous Afap1l2 protein in mouse prostate lysates by Western Blot at a 1:500 dilution (Lot 54527).
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Tamara (Verified Customer) (12-02-2020) | antibody works very well in detecting endogenous Afap1l2 protein in mouse prostate lysates
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