Tested Applications
| Positive WB detected in | human plasma |
| Positive IP detected in | human plasma tissue |
| Positive IHC detected in | mouse brain tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66113-1-Ig targets AFM in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16866 Product name: Recombinant human AFM protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 264-599 aa of BC109020 Sequence: SNYDGCCEGDVVQCIRDTSKVMNHICSKQDSISSKIKECCEKKIPERGQCIINSNKDDRPKDLSLREGKFTDSENVCQERDADPDTFFAKFTFEYSRRHPDLSIPELLRIVQIYKDLLRNCCNTENPPGCYRYAEDKFNETTEKSLKMVQQECKHFQNLGKDGLKYHYLIRLTKIAPQLSTEELVSLGEKMVTAFTTCCTLSEEFACVDNLADLVFGELCGVNENRTINPAVDHCCKTNFAFRRPCFESLKADKTYVPPPFSQDLFTFHADMCQSQNEELQRKTDRFLVNLVKLKHELTDEELQSLFTNFANVVDKCCKAESPEVCFNEESPKIGN Predict reactive species |
| Full Name | afamin |
| Calculated Molecular Weight | 599 aa, 69 kDa |
| Observed Molecular Weight | 85 kDa |
| GenBank Accession Number | BC109020 |
| Gene Symbol | AFM |
| Gene ID (NCBI) | 173 |
| RRID | AB_2881512 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P43652 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
AFM (Afamin) is a member of the albumin superfamily, which comprises albumin, vitamin D-binding protein, a-fetoprotein, and afamin. AFM is present in the plasma/serum, cerebrospinal fluid, and follicular fluid, and functions as a vitamin E-binding protein. Alterations of expression level of AFM have been linked to variety of diseases like cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for AFM antibody 66113-1-Ig | Download protocol |
| IHC protocol for AFM antibody 66113-1-Ig | Download protocol |
| IP protocol for AFM antibody 66113-1-Ig | Download protocol |
| WB protocol for AFM antibody 66113-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The AFM Monoclonal antibody (2G9F6), catalog number 66113-1-Ig, has a total of 1 citation. It appears in the Journal of Ginseng Research (J Ginseng Res, IF 7.5), published in 2017. The cited study used a proteomic approach to reveal differential protein expression in Drosophila melanogaster treated with red ginseng extract (Panax ginseng), applying the antibody for Western blot analysis. The research field is related to proteomics and ginseng pharmacology.
| Species | Application | Title |
|---|---|---|
J Ginseng Res A proteomic approach reveals the differential protein expression in Drosophila melanogaster treated with red ginseng extract (Panax ginseng) |
