AFM Monoclonal antibody

AFM Monoclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2G9F6

Cat no : 66113-1-Ig

Synonyms

afamin, afamin/AFM, AFM, ALB2, ALBA, ALF, Alpha Alb, Alpha albumin



Tested Applications

Positive WB detected inhuman plasma
Positive IP detected inhuman plasma tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66113-1-Ig is AFM Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AFM fusion protein Ag16866
Full Name afamin
Calculated molecular weight 599 aa, 69 kDa
Observed molecular weight 85 kDa
GenBank accession numberBC109020
Gene symbol AFM
Gene ID (NCBI) 173
RRIDAB_2881512
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AFM (Afamin) is a member of the albumin superfamily, which comprises albumin, vitamin D-binding protein, a-fetoprotein, and afamin. AFM is present in the plasma/serum, cerebrospinal fluid, and follicular fluid, and functions as a vitamin E-binding protein. Alterations of expression level of AFM have been linked to variety of diseases like cancer.

Protocols

Product Specific Protocols
WB protocol for AFM antibody 66113-1-IgDownload protocol
IHC protocol for AFM antibody 66113-1-IgDownload protocol
IF protocol for AFM antibody 66113-1-IgDownload protocol
IP protocol for AFM antibody 66113-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols