AFMID Polyclonal antibody

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  WB analysis of mouse kidney using 19522-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19522-1-AP (AFMID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 19522-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19522-1-AP (AFMID antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 19522-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 19522-1-AP (AFMID antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 19522-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19522-1-AP (AFMID antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 19522-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19522-1-AP (AFMID antibody) at dilution of 1:100 (under 40x lens).

• Featured Product
• KD/KO Validated

AFMID Polyclonal Antibody for WB, IHC, ELISA

Cat No. 19522-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

AFMID, arylformamidase, KF, Kynurenine formamidase, Probable arylformamidase

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Tested Applications

• Positive WB detected in: mouse kidney tissue, mouse liver tissue, human liver tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below

Product Information

19522-1-AP targets AFMID in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: arylformamidase
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: NM_001145526
• Gene Symbol: AFMID
• Gene ID (NCBI): 125061
• RRID: AB_11041712
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q63HM1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AFMID,also named as KF, belongs to the AFMID family. AFMID catalyzes the hydrolysis of N-formyl-L-kynurenine to L-kynurenine, the second step in the conversion of tryptophan to nicotinic acid, NAD(H) and NADP(H). AFMID is required for elimination of toxic metabolites.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human, mouse	WB	Cancer Cell; Inhibition of De Novo NAD(+) Synthesis by Oncogenic URI Causes Liver Tumorigenesis through DNA Damage.; Authors - Krishna S Tummala ; KD Validated
human	WB	Genome Res; A human-specific switch of alternatively spliced AFMID isoforms contributes to TP53 mutations and tumor recurrence in hepatocellular carcinoma.; Authors - Kuan-Ting Lin
mouse	WB	Mol Cell Endocrinol; Hepatic NAD+levels and NAMPT abundance are unaffected during prolonged high-fat diet consumption in C57BL/6JBomTac mice.; Authors - Morten Dall
human	WB	J Proteome Res; Proteomics Analysis of Renal Cell Line Caki-2 with AFMID Overexpression and Potential Biomarker Discovery in Urine; Authors - Jiameng Sun