AFMID Polyclonal antibody

• Featured Product
• KD/KO Validated

AFMID Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 19522-1-AP

Synonyms

AFMID, arylformamidase, KF, Kynurenine formamidase, Probable arylformamidase

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Validation Data Gallery

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  WB analysis of mouse kidney using 19522-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19522-1-AP (AFMID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 19522-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19522-1-AP (AFMID antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 19522-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 19522-1-AP (AFMID antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 19522-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19522-1-AP (AFMID antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 19522-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19522-1-AP (AFMID antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, mouse liver tissue, human liver tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below

Product Information

19522-1-AP targets AFMID in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: arylformamidase
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: NM_001145526
• Gene Symbol: AFMID
• Gene ID (NCBI): 125061
• RRID: AB_11041712
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AFMID,also named as KF, belongs to the AFMID family. AFMID catalyzes the hydrolysis of N-formyl-L-kynurenine to L-kynurenine, the second step in the conversion of tryptophan to nicotinic acid, NAD(H) and NADP(H). AFMID is required for elimination of toxic metabolites.

Publications

Species	Application	Title
human, mouse	WB	Cancer Cell; Inhibition of De Novo NAD(+) Synthesis by Oncogenic URI Causes Liver Tumorigenesis through DNA Damage.; Authors - Krishna S Tummala ; KD Validated
human	WB	Genome Res; A human-specific switch of alternatively spliced AFMID isoforms contributes to TP53 mutations and tumor recurrence in hepatocellular carcinoma.; Authors - Kuan-Ting Lin
mouse	WB	Mol Cell Endocrinol; Hepatic NAD+levels and NAMPT abundance are unaffected during prolonged high-fat diet consumption in C57BL/6JBomTac mice.; Authors - Morten Dall
human	WB	J Proteome Res; Proteomics Analysis of Renal Cell Line Caki-2 with AFMID Overexpression and Potential Biomarker Discovery in Urine; Authors - Jiameng Sun